Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI6C1

Concentration

1 mg/ml

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Product Summary for RIG-I Antibody (OTI6C1)

Immunogen

Full length human recombinant protein of human DDX58(NP_055129) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

106.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RIG-I Antibody (OTI6C1)

Western Blot: RIG-I Antibody (OTI6C1) [NBP2-45678]

Western Blot: RIG-I Antibody (OTI6C1) [NBP2-45678]

Western Blot: RIG-I Antibody (OTI6C1) [NBP2-45678] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIG-I.
Immunocytochemistry/ Immunofluorescence: RIG-I Antibody (OTI6C1) [NBP2-45678]

Immunocytochemistry/ Immunofluorescence: RIG-I Antibody (OTI6C1) [NBP2-45678]

Immunocytochemistry/Immunofluorescence: RIG-I Antibody (OTI6C1) [NBP2-45678] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY RIG-I.
Immunohistochemistry: RIG-I Antibody (OTI6C1) [NBP2-45678]

Immunohistochemistry: RIG-I Antibody (OTI6C1) [NBP2-45678]

Immunohistochemistry: RIG-I Antibody (OTI6C1) [NBP2-45678] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Applications for RIG-I Antibody (OTI6C1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:4000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RIG-I

RIG-I, also known as DDX58, consists of a 106.6 kDa and a 101.4 kDa isoform and is involved in recognizing viruses and activating a response. Current research is being conducted with diseases and disorders such as hepatitis, rubella, influenza, rabies, hemorrhagic fever, lupus nephritis, and tick-borne encephalitis. The protein interacts in pathways such as NF-kappa signaling, cytosolic DNA-sensing, measles, influenza A, and mediated induction pathways with proteins such as CYLD, ZC3HAV1, DDX3X, MAVS, and WRNIP1.

Long Name

RIG-I

Alternate Names

DDX58, RIGI

Gene Symbol

RIGI

Additional RIG-I Products

Product Documents for RIG-I Antibody (OTI6C1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RIG-I Antibody (OTI6C1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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