RING1 Antibody (JE34-66)

Western Blot: RING1 Antibody (JE34-66) [NBP3-32904]

Western Blot: RING1 Antibody (JE34-66) [NBP3-32904] - Western blot analysis of RING1 on different lysates with Rabbit anti-RING1 antibody (NBP3-32904) at 1/1,000 dilution.

Lane 1: THP-1 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: 22RV1 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 42 kDa
Observed band size: 50 kDa

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32904) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: RING1 Antibody (JE34-66) [NBP3-32904]

Immunohistochemistry: RING1 Antibody (JE34-66) [NBP3-32904] - Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-RING1 antibody (NBP3-32904) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32904) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.