RIOK3 Antibody (3G11)
Novus Biologicals, part of Bio-Techne | Catalog # H00008780-M02
Key Product Details
Species Reactivity
Human
Applications
ELISA, Knockdown Validated, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 3G11
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
RIOK3 (AAH39729, 411 a.a. ~ 516 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NMLWHAGKVWLIDVSQSVEPTHPHGLEFLFRDCRNVSQKGGVKEALSERELFNAVSGLNITADNEADFLAEIEALEKMNEDHVQKNGRKAASFLKDDGDPPLLYDE
Specificity
RIOK3 - RIO kinase 3 (yeast)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for RIOK3 Antibody (3G11)
![Western Blot: RIOK3 Antibody (3G11) [H00008780-M02]](https://resources.bio-techne.com/images/products/RIOK3-Antibody-3G11-Western-Blot-H00008780-M02-img0007.jpg "Western Blot: RIOK3 Antibody (3G11) [H00008780-M02]")
Western Blot: RIOK3 Antibody (3G11) [H00008780-M02]
Western Blot: RIOK3 Antibody (3G11) [H00008780-M02] - RIOK3 monoclonal antibody (M02), clone 3G11 Analysis of RIOK3 expression in K-562.![Western Blot: RIOK3 Antibody (3G11) [H00008780-M02]](https://resources.bio-techne.com/images/products/RIOK3-Antibody-3G11-Western-Blot-H00008780-M02-img0005.jpg "Western Blot: RIOK3 Antibody (3G11) [H00008780-M02]")
Western Blot: RIOK3 Antibody (3G11) [H00008780-M02]
Western Blot: RIOK3 Antibody (3G11) [H00008780-M02] - Analysis of RIOK3 over-expressed 293 cell line, cotransfected with RIOK3 Validated Chimera RNAi ( Cat # H00008780-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RIOK3 monoclonal antibody (M02) clone 3G11 (Cat # H00008780-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.![Western Blot: RIOK3 Antibody (3G11) [H00008780-M02]](https://resources.bio-techne.com/images/products/RIOK3-Antibody-3G11-Western-Blot-H00008780-M02-img0008.jpg "Western Blot: RIOK3 Antibody (3G11) [H00008780-M02]")
Western Blot: RIOK3 Antibody (3G11) [H00008780-M02]
Western Blot: RIOK3 Antibody (3G11) [H00008780-M02] - Analysis of RIOK3 expression in transfected 293T cell line by RIOK3 monoclonal antibody (M02), clone 3G11.Lane 1: RIOK3 transfected lysate(59.1 KDa).Lane 2: Non-transfected lysate.Applications for RIOK3 Antibody (3G11)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: RIOK3
Alternate Names
DKFZp779L1370, EC 2.7.11.1, homolog of the Aspergillus nidulans sudD gene product, RIO kinase 3, RIO kinase 3 (yeast), serine/threonine-protein kinase RIO3, sudD (suppressor of bimD6, Aspergillus nidulans) homolog, sudD homolog, sudD suppressor of bimD6 homolog, SUDDsudD suppressor of Aspergillus nidulans bimD6 homolog
Entrez Gene IDs
8780 (Human)
Gene Symbol
RIOK3
OMIM
603579 (Human)
UniProt
Additional RIOK3 Products
Product Documents for RIOK3 Antibody (3G11)
Product Specific Notices for RIOK3 Antibody (3G11)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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