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RIOK3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86982

Novus Biologicals, part of Bio-Techne
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NBP1-86982
NBP1-86982-25ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IPQNTISCSLADVMSEQLAKELQLEEEAAVFPEVAVAEGPFITGENIDTSSDLMLAQMLQMEYDREYDAQLRREEKKFNGDSKVSISFENYRKVHPYEDSDSSEDEVDWQDTRDDPYRPAKP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RIOK3 Antibody

Western Blot: RIOK3 Antibody [NBP1-86982]

Western Blot: RIOK3 Antibody [NBP1-86982]

Western Blot: RIOK3 Antibody [NBP1-86982] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunocytochemistry/ Immunofluorescence: RIOK3 Antibody [NBP1-86982]

Immunocytochemistry/ Immunofluorescence: RIOK3 Antibody [NBP1-86982]

Immunocytochemistry/Immunofluorescence: RIOK3 Antibody [NBP1-86982] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane, cytosol & the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RIOK3 Antibody [NBP1-86982]

Immunohistochemistry-Paraffin: RIOK3 Antibody [NBP1-86982]

Immunohistochemistry-Paraffin: RIOK3 Antibody [NBP1-86982] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.

Applications for RIOK3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RIOK3

RIOK3 was identified by the similarity of its product to the Aspergillus nidulans SUDD protein, an extragenic suppressor of the heat-sensitive bimD6 mutation that fails to attach properly to the spindle microtubules at a restrictive temperature. The specific function of this gene has not yet been determined.

Alternate Names

DKFZp779L1370, EC 2.7.11.1, homolog of the Aspergillus nidulans sudD gene product, RIO kinase 3, RIO kinase 3 (yeast), serine/threonine-protein kinase RIO3, sudD (suppressor of bimD6, Aspergillus nidulans) homolog, sudD homolog, sudD suppressor of bimD6 homolog, SUDDsudD suppressor of Aspergillus nidulans bimD6 homolog

Gene Symbol

RIOK3

Additional RIOK3 Products

Product Documents for RIOK3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RIOK3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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