RIPK1/RIP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56160

Novus Biologicals, part of Bio-Techne
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NB100-56160
NB100-56160SS
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all RIPK1/RIP1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to amino acids 269-287 (EANPEARPTFPGIEEKFRP) of human RIP was used as immunogen, GenBank no. AAC32232.1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RIPK1/RIP1 Antibody

Immunohistochemistry-Paraffin: RIPK1/RIP1 Antibody [NB100-56160]

Immunohistochemistry-Paraffin: RIPK1/RIP1 Antibody [NB100-56160]

Immunohistochemistry-Paraffin: RIPK1/RIP1 Antibody [NB100-56160] - Analysis of tonsil using this antibody at 1:2000 in (A) low magnification overview of a secondary follicle. (A1) and (A2), sucessively higher magnifications of the germinal center from (A). Hematoxylin-eosin counterstain.
Immunohistochemistry-Paraffin: RIPK1/RIP1 Antibody [NB100-56160]

Immunohistochemistry-Paraffin: RIPK1/RIP1 Antibody [NB100-56160]

Immunohistochemistry-Paraffin: RIPK1/RIP1 Antibody [NB100-56160] - Tissue sections using NB100-56160 at 1:2000 of (A) normal human breast and (B) normal human colon. Plasma cells in the lamina propia of the colon are stained. Hematoxylin-eosin counterstain.

Applications for RIPK1/RIP1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000-1:5000

Western Blot

1:1000-1:2000
Application Notes
Immunoprecipitation, Western Blot, Immunohistochemistry-Paraffin IHC (frozen): Users should optimize according to model and immunodetection system used (secondary reagents)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RIPK1/RIP1

RIP (Receptor Interacting Protein) is a 74 kD Ser/Thr kinase which interacts with CD95 (Fas/APO1) receptor and the tumor necrosis factor receptor (TNFR1). It is a cell death domain adapter protein which can bind to the adapter proteins TRADD, RAID (CRADD) and TRAF2. RIP contains an N terminal region with homology to protein kinases, an intermediate domain capable of association with MAPKKK and a C terminal region containing an intracellular death domain motif. RIP activates both p38 MAP Kinase and SAPK families. In vitro, RIP induces apoptosis, as well as SAPK/JNK and NF-kB activation. NF-kB activation through TRADD, TRAF2 and RIP can be triggered also by DR3/APO3 upon activation with APO3/Tweak ligand. DR4 and DR5 also use FADD, TRADD, and RIP in their signal transduction pathways. RIP deficient mice fail to thrive, displaying extensive apoptosis in both lymphoid and adipose tissues and dying at 1-3 days of age. RIP possesses kinase activity as it autophosphorylates itself on serine and threonine residues.

Long Name

Receptor (TNFRSF)-Interacting Serine-Threonine Kinase 1

Alternate Names

RIP, RIP1

Entrez Gene IDs

8737 (Human)

Gene Symbol

RIPK1

UniProt

Q13546

Additional RIPK1/RIP1 Products

Product Documents for RIPK1/RIP1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RIPK1/RIP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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