RIPK2 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32424
![Western Blot: RIPK2 AntibodyBSA Free [NBP1-32424]](https://resources.bio-techne.com/images/products/RIPK2-Antibody-Western-Blot-NBP1-32424-img0009.jpg "Western Blot: RIPK2 AntibodyBSA Free [NBP1-32424]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Summary for RIPK2 Antibody - BSA Free
Immunogen
Recombinant protein encompassing a sequence within the center region of human RIP2. The exact sequence is proprietary.
Reactivity Notes
Bovine (80%).
Localization
Cytoplasm
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for RIPK2 Antibody - BSA Free
Western Blot: RIPK2 AntibodyBSA Free [NBP1-32424]
Western Blot: RIPK2 Antibody [NBP1-32424] - Total protein from human HeLa, A431 and Raji cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-RIPK2 (NBP1-32424) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).![Immunocytochemistry/ Immunofluorescence: RIPK2 Antibody - BSA Free [NBP1-32424]](https://resources.bio-techne.com/images/products/RIPK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32424-img0008.jpg "Immunocytochemistry/ Immunofluorescence: RIPK2 Antibody - BSA Free [NBP1-32424]")
Immunocytochemistry/ Immunofluorescence: RIPK2 Antibody - BSA Free [NBP1-32424]
Immunocytochemistry/Immunofluorescence: RIPK2 Antibody [NBP1-32424] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-RIPK2 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.![Immunohistochemistry-Paraffin: RIPK2 Antibody - BSA Free [NBP1-32424]](https://resources.bio-techne.com/images/products/RIPK2-Antibody-Immunohistochemistry-Paraffin-NBP1-32424-img0007.jpg "Immunohistochemistry-Paraffin: RIPK2 Antibody - BSA Free [NBP1-32424]")
Immunohistochemistry-Paraffin: RIPK2 Antibody - BSA Free [NBP1-32424]
Immunohistochemistry-Paraffin: RIPK2 Antibody [NBP1-32424] - Analysis of a FFPE tissue section of human placenta using 1:200 dilution of RIPK2 (NBP1-32424) antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This RIPK2 antibody generated cytoplasmic reactivity in most trophoblastic cells.Applications for RIPK2 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RIPK2/RIP2
Long Name
Receptor Interacting Serine/Threonine Kinase 2
Alternate Names
CARD3, GIG30, RICK, RIP-2, RIP2
Gene Symbol
RIPK2
Additional RIPK2/RIP2 Products
Product Documents for RIPK2 Antibody - BSA Free
Product Specific Notices for RIPK2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.