RNA Polymerase II/POLR2A Antibody (4H8) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80928

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Primate (Negative), Yeast

Applications

Chromatin Immunoprecipitation (ChIP), CyTOF-ready, ELISA, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4H8

Format

Azide and BSA Free

Concentration

1 mg/ml

View all RNA Polymerase II/POLR2A Antibodies »

Product Specifications

Immunogen

10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser 5 YSPTSpPS (Human). [UniProt# P24928]

Reactivity Notes

Does not cross react with monkey.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

217 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RNA Polymerase II/POLR2A Antibody (4H8) - Azide and BSA Free

Western Blot: RNA Polymerase II/POLR2A Antibody (4H8)Azide and BSA Free [NBP2-80928]

Western Blot: RNA Polymerase II/POLR2A Antibody (4H8)Azide and BSA Free [NBP2-80928]

Western Blot: RNA Polymerase II/POLR2A Antibody (4H8) - Azide and BSA Free [NBP2-80928] - Whole cell protein from HeLa (lane 1), MCF7 (lane 2), NIH-3T3 (lane 3) and Cos7 (lane 4) were separated by SDS-PAGE on a 6% gel, transferred to PVDF and probed with 2 ug/mL NB200-598. RNA Pol II reactivity (arrowhead) is observed in HeLa, MCF and 3T3 cells. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A Antibody (4H8) - Azide and BSA Free [NBP2-80928]

Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A Antibody (4H8) - Azide and BSA Free [NBP2-80928]

Immunocytochemistry/Immunofluorescence: RNA Polymerase II/POLR2A Antibody (4H8) - Azide and BSA Free [NBP2-80928] - RNA Polymerase II/POLR2A antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image from the standard format of this antibody.
Flow Cytometry: RNA Polymerase II/POLR2A Antibody (4H8) - Azide and BSA Free [NBP2-80928]

Flow Cytometry: RNA Polymerase II/POLR2A Antibody (4H8) - Azide and BSA Free [NBP2-80928]

Flow Cytometry: RNA Polymerase II/POLR2A Antibody (4H8) - Azide and BSA Free [NBP2-80928] - An intracellular stain was performed on HeLa cells with RNA Polymerase II/POLR2A (4H8) antibody NB200-598PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes. Both antibodies were directly conjugated to phycoerythrin.
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Applications for RNA Polymerase II/POLR2A Antibody (4H8) - Azide and BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

ELISA

1:100 - 1:2000

Flow Cytometry

1 ug per million cells

Immunocytochemistry/ Immunofluorescence

1:100 - 1:1000

Immunoprecipitation

1:10 - 1:500

Western Blot

1 - 2 ug/ml
Application Notes
In WB a band can be seen at approximately 217 kDa. Optimal dilutions/concentrations should be determined by the end user. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RNA Polymerase II/POLR2A

RNA polymerase II (RNAPII) is a 12-subunit enzyme which synthesizes all mRNAs as well as several non-coding RNAs in eukaryotic cells thereby playing a central role in cellular functions. RNAPII is a DNA-dependent RNA polymerase enzyme which catalyzes the transcription of DNA into RNA employing four ribonucleoside triphosphates as substrates. The activity of RNAPII transcription machinery is regulated by DNA-binding transcriptional regulators in a gene/cell type-specific manner, whereas general transcription factors act as RNAPII accessory proteins required for the transcription of most class II genes, and the co-activators/co-repressors serve as bridges between DNA-bound factors and the RNAPII machinery, some affecting the organization and/or chemical modification of the chromatin template of RNAPII. RNA polymerase II subunit B1 (POLR2A) is the largest and the catalytic component of RNAPII which forms the polymerase active center together with the second largest subunit and is the central component of the basal RNAPII transcription machinery. It is composed of mobile elements that move relative to each other and POLR2A is the part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template.

Long Name

DNA-directed RNA Polymerase II Subunit RPB1

Alternate Names

POLR2, POLR2A, POLRA, RPB1, RPO2, RPOL2

Gene Symbol

POLR2A

Additional RNA Polymerase II/POLR2A Products

Product Documents for RNA Polymerase II/POLR2A Antibody (4H8) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RNA Polymerase II/POLR2A Antibody (4H8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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