RNase L Antibody (2E9) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80929
![Western Blot: RNase L Antibody (2E9)Azide and BSA Free [NBP2-80929]](https://resources.bio-techne.com/images/products/RNase-L-Antibody-2E9-Azide-and-BSA-Free-Western-Blot-NBP2-80929-img0001.jpg "Western Blot: RNase L Antibody (2E9)Azide and BSA Free [NBP2-80929]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Hamster, Mouse (Negative), Primate, Rat (Negative)
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 2E9
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for RNase L Antibody (2E9) - Azide and BSA Free
Immunogen
Purified full-length recombinant human RNaseL produced in insect cells. [UniProt# Q05823]
Reactivity Notes
This antibody does not react with mouse or rat.
Localization
Cytoplasmic and Mitochondrial.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
83 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for RNase L Antibody (2E9) - Azide and BSA Free
Western Blot: RNase L Antibody (2E9)Azide and BSA Free [NBP2-80929]
Western Blot: RNase L Antibody (2E9) - Azide and BSA Free [NBP2-80929] - RNaseL in insect cell extracts transfected with recombinant human RNase L (0.1 ug) and Hey1B (100 ug) Image from the standard format of this antibody.![Immunohistochemistry: RNase L Antibody (2E9) - Azide and BSA Free [NBP2-80929]](https://resources.bio-techne.com/images/products/RNase-L-Antibody-2E9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80929-img0002.jpg "Immunohistochemistry: RNase L Antibody (2E9) - Azide and BSA Free [NBP2-80929]")
Immunohistochemistry: RNase L Antibody (2E9) - Azide and BSA Free [NBP2-80929]
Immunohistochemistry: RNase L Antibody (2E9) - Azide and BSA Free [NBP2-80929] - RNase L antibody was tested in human colon using DAB with hematoxylin counterstain. Image from the standard format of this antibody.Applications for RNase L Antibody (2E9) - Azide and BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunohistochemistry
1:200-1:1000
Immunohistochemistry-Paraffin
1:200-1:1000
Western Blot
1:1000-1:4000
Application Notes
This RNase L (2E9) antibody is useful for ELISA, Western Blot and Immunohistochemistry on paraffin-embedded sections. By WB, this antibody recognizes a band at ~83 kDa, representing RNase L. A low-molecular mass, 37 kDa, isoform of RNase L has been described in peripheral blood mononuclear cell extracts. In IHC-P, staining was observed in the cytoplasm of human colon cells. Prior to immunostaining paraffin tissues, antigen retrieval with Tris-EDTA buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: RNase L
Alternate Names
2-5A-dependent ribonuclease, 2-5A-dependent RNase, 2'-5'-oligoisoadenylate synthetase-dependent, EC 3.1.26.-, HPC1, interferon-induced 2-5A-dependent RNase, MGC104972, MGC133329, PRCA1, prostate cancer 1, Ribonuclease 4, Ribonuclease L, ribonuclease L (2'-5'-oligoisoadenylate synthetase-dependent), RNase L, RNS4DKFZp781D08126
Gene Symbol
RNASEL
Additional RNase L Products
Product Documents for RNase L Antibody (2E9) - Azide and BSA Free
Product Specific Notices for RNase L Antibody (2E9) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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