RNF125 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-78703

Novus Biologicals, part of Bio-Techne
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NBP2-78703
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all RNF125 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-RSLLEYVNHSNTT corresponding to C-Terminus according to NP_060301.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for RNF125 Antibody

Immunocytochemistry/ Immunofluorescence: RNF125 Antibody [NBP2-78703]

Immunocytochemistry/ Immunofluorescence: RNF125 Antibody [NBP2-78703]

Immunocytochemistry/Immunofluorescence: RNF125 Antibody [NBP2-78703] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing endoplasmic reticulum/Golgi and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry: RNF125 Antibody [NBP2-78703]

Flow Cytometry: RNF125 Antibody [NBP2-78703]

Flow Cytometry: RNF125 Antibody [NBP2-78703] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for RNF125 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:8000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RNF125

RNF125 encodes a novel E3 ubiquitin ligase that contains an N-terminal RING finger domain. The encoded protein may function as a positive regulator in the T-cell receptor signaling pathway.

Alternate Names

E3 ubiquitin-protein ligase RNF125, EC 6.3.2.-, FLJ20456, MGC21737, ring finger protein 125TRAC1, T-cell RING activation protein 1, T-cell ring protein identified in activation screen, TRAC-1

Gene Symbol

RNF125

Additional RNF125 Products

Product Documents for RNF125 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RNF125 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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