RNF20 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2243
![Immunoprecipitation: RNF20 Antibody [NB100-2243]](https://resources.bio-techne.com/images/products/RNF20-Antibody-Immunoprecipitation-NB100-2243-img0010.jpg "Immunoprecipitation: RNF20 Antibody [NB100-2243]")
![Western Blot: RNF20 Antibody [NB100-2243]](https://resources.bio-techne.com/images/products/RNF20-Antibody-Western-Blot-NB100-2243-img0009.jpg "Western Blot: RNF20 Antibody [NB100-2243]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (100%), Chicken (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 125 and 175 of human Ring Finger 20 using the numbering given in entry NP_062538.5 (GeneID 56254).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for RNF20 Antibody - BSA Free
Western Blot: RNF20 Antibody [NB100-2243]
Western Blot: RNF20 Antibody [NB100-2243] - Detection of Human RNF20 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RNF20 antibody NB100-2243 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.![Immunohistochemistry-Paraffin: RNF20 Antibody [NB100-2243]](https://resources.bio-techne.com/images/products/RNF20-Antibody-Immunohistochemistry-NB100-2243-img0006.jpg "Immunohistochemistry-Paraffin: RNF20 Antibody [NB100-2243]")
Immunohistochemistry-Paraffin: RNF20 Antibody [NB100-2243]
Immunohistochemistry-Paraffin: RNF20 Antibody [NB100-2243] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RNF20 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB![Immunohistochemistry-Paraffin: RNF20 Antibody [NB100-2243]](https://resources.bio-techne.com/images/products/RNF20-Antibody-Immunohistochemistry-NB100-2243-img0005.jpg "Immunohistochemistry-Paraffin: RNF20 Antibody [NB100-2243]")
Immunohistochemistry-Paraffin: RNF20 Antibody [NB100-2243]
Immunohistochemistry-Paraffin: RNF20 Antibody [NB100-2243] - Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti- RNF20 used at a dilution of 1:1,000 (1ug/ml). Detection: DABApplications for RNF20 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:2000
Immunohistochemistry-Paraffin
1:500 - 1:2000
Immunoprecipitation
2- 10 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: RNF20
Alternate Names
BRE1, BRE1 E3 ubiquitin ligase homolog, BRE1-A, BRE1AMGC129667, E3 ubiquitin-protein ligase BRE1A, EC 6.3.2, EC 6.3.2.-, FLJ11189, FLJ20382, hBRE1MGC129668, homolog of S. cerevisiae BRE1, KAIA2779, ring finger protein 20KIAA2779
Entrez Gene IDs
56254 (Human)
Gene Symbol
RNF20
UniProt
Additional RNF20 Products
Product Documents for RNF20 Antibody - BSA Free
Product Specific Notices for RNF20 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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