RNF213 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88466
![Immunohistochemistry-Paraffin: RNF213 Antibody [NBP1-88466]](https://resources.bio-techne.com/images/products/RNF213-Antibody-Immunohistochemistry-Paraffin-NBP1-88466-img0013.jpg "Immunohistochemistry-Paraffin: RNF213 Antibody [NBP1-88466]")
Key Product Details
Species Reactivity
Validated:
Human, Xenopus
Cited:
Frog - Xenopus (African Clawed Frog)
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VGKNEQGEPEDLKKPEGKNRSAAAVKNEKEQKNQEADVQEVKASTLSPGGGVTVFFHAIISLHFPFNPDLHKVFIRGGEEFGESKWDSNICELHYTRDLGHDRVLVEGIVCISKKHLDKYIPYKYVIYNGESFEYEFIYKH
Reactivity Notes
Xenopus reactivity reported in scientific literature (PMID: 30979826).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for RNF213 Antibody
![Western Blot: RNF213 Antibody [NBP1-88466]](https://resources.bio-techne.com/images/products/RNF213-Antibody-Western-Blot-NBP1-88466-img0017.jpg "Western Blot: RNF213 Antibody [NBP1-88466]")
Western Blot: RNF213 Antibody [NBP1-88466]
Western Blot: RNF213 Antibody [NBP1-88466] - TRAIP activity is required for Mcm7 ubiquitylation in mitosis. The same experiment from Fig 3B but with addition of the RNF213 blot. An asterisk "*" by the Psf2 blot indicates the band of cyclin A1delta that is recognized by the Psf2 antibody. Image collected and cropped by CiteAb from the following publication (https://www.life-science-alliance.org/lookup/doi/10.26508/lsa.201900390), licensed under a CC-BY license.![Immunocytochemistry/ Immunofluorescence: RNF213 Antibody [NBP1-88466]](https://resources.bio-techne.com/images/products/RNF213-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88466-img0007.jpg "Immunocytochemistry/ Immunofluorescence: RNF213 Antibody [NBP1-88466]")
Immunocytochemistry/ Immunofluorescence: RNF213 Antibody [NBP1-88466]
Immunocytochemistry/Immunofluorescence: RNF213 Antibody [NBP1-88466] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: RNF213 Antibody [NBP1-88466]](https://resources.bio-techne.com/images/products/RNF213-Antibody-Immunohistochemistry-Paraffin-NBP1-88466-img0016.jpg "Immunohistochemistry-Paraffin: RNF213 Antibody [NBP1-88466]")
Immunohistochemistry-Paraffin: RNF213 Antibody [NBP1-88466]
Immunohistochemistry-Paraffin: RNF213 Antibody [NBP1-88466] - Staining of human rectum shows moderate cytoplasmic positivity in glandular cells.![Immunohistochemistry-Paraffin: RNF213 Antibody [NBP1-88466]](https://resources.bio-techne.com/images/products/RNF213-Antibody-Immunohistochemistry-Paraffin-NBP1-88466-img0014.jpg "Immunohistochemistry-Paraffin: RNF213 Antibody [NBP1-88466]")
![Immunohistochemistry-Paraffin: RNF213 Antibody [NBP1-88466]](https://resources.bio-techne.com/images/products/RNF213-Antibody-Immunohistochemistry-Paraffin-NBP1-88466-img0015.jpg "Immunohistochemistry-Paraffin: RNF213 Antibody [NBP1-88466]")
Applications for RNF213 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
Image collected and cropped by CiteAb from the following publication (http://www.life-science-alliance.org/lookup/doi/10.26508/lsa.201900390), licensed under a CC-BY license.
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RNF213
Alternate Names
ALK lymphoma oligomerization partner on chromosome 17, C17orf27, chromosome 17 open reading frame 27, DKFZp762N1115, FLJ13051, KIAA1554MGC46622, KIAA1618, MGC9929, NET57, protein ALO17, ring finger protein 213
Gene Symbol
RNF213
Additional RNF213 Products
Product Documents for RNF213 Antibody
Product Specific Notices for RNF213 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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