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RNF25 Antibody (1D10)

Novus Biologicals, part of Bio-Techne | Catalog # H00064320-M07

Novus Biologicals, part of Bio-Techne
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H00064320-M07

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1D10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

RNF25 (NP_071898.2, 352 a.a. ~ 459 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RRHPKGGECHAPKGTRDTQELPPPEGPLKEPMDLKPEPHSQGVEGPPQEKGPGSWQGPPPRRTRDCVRWERSKGRTPGSSYPRLPRGQGAYRPGTRRESLGLESKDGS

Specificity

RNF25 - ring finger protein 25 (1D10)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for RNF25 Antibody (1D10)

Immunohistochemistry-Paraffin: RNF25 Antibody (1D10) [H00064320-M07]

Immunohistochemistry-Paraffin: RNF25 Antibody (1D10) [H00064320-M07]

Immunohistochemistry-Paraffin: RNF25 Antibody (1D10) [H00064320-M07] - Analysis of monoclonal antibody to RNF25 on human epithelium tissue. Image from verified customer review.
Immunohistochemistry-Paraffin: RNF25 Antibody (1D10) [H00064320-M07]

Immunohistochemistry-Paraffin: RNF25 Antibody (1D10) [H00064320-M07]

Immunohistochemistry-Paraffin: RNF25 Antibody (1D10) [H00064320-M07] - Analysis of monoclonal antibody to RNF25 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml
Sandwich ELISA: RNF25 Antibody (1D10) [H00064320-M07] - Detection limit for recombinant GST tagged RNF25 is 0.03 ng/ml as a capture antibody.

Applications for RNF25 Antibody (1D10)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using H00064320-M07 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RNF25

The protein encoded by this gene contains a RING finger motif. The mouse counterpart of this protein has been shown to interact with Rela, the p65 subunit of NF-kappaB (NFKB), and modulate NFKB-mediated transcription activity. The mouse protein also binds ubiquitin-conjugating enzymes (E2s) and is a substrate for E2-dependent ubiquitination. [provided by RefSeq]

Alternate Names

AO7, EC 6.3.2.-, FLJ13906, ring finger protein 25E3 ubiquitin-protein ligase RNF25, ring finger protein AO7

Entrez Gene IDs

64320 (Human)

Gene Symbol

RNF25

Additional RNF25 Products

Product Documents for RNF25 Antibody (1D10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RNF25 Antibody (1D10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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