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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI5E10

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human RNF39(NP_079512) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

45.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RNF39 Antibody (OTI5E10) - Azide and BSA Free

Western Blot: RNF39 Antibody (OTI5E10)Azide and BSA Free [NBP2-73927]

Western Blot: RNF39 Antibody (OTI5E10)Azide and BSA Free [NBP2-73927]

Western Blot: RNF39 Antibody (OTI5E10) - Azide and BSA Free [NBP2-73927] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF39.
Immunocytochemistry/ Immunofluorescence: RNF39 Antibody (OTI5E10) - Azide and BSA Free [NBP2-73927]

Immunocytochemistry/ Immunofluorescence: RNF39 Antibody (OTI5E10) - Azide and BSA Free [NBP2-73927]

Immunocytochemistry/Immunofluorescence: RNF39 Antibody (OTI5E10) - Azide and BSA Free [NBP2-73927] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY RNF39.
Immunohistochemistry: RNF39 Antibody (OTI5E10) - Azide and BSA Free [NBP2-73927]

Immunohistochemistry: RNF39 Antibody (OTI5E10) - Azide and BSA Free [NBP2-73927]

Immunohistochemistry: RNF39 Antibody (OTI5E10) - Azide and BSA Free [NBP2-73927] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Applications for RNF39 Antibody (OTI5E10) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:200

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RNF39

RNF39 lies within the major histocompatibility complex class I region on chromosome 6. Studies of a similar rat protein suggest that this gene encodes a protein that plays a role in an early phase of synaptic plasticity. Alternative splicing results in three transcript variants encoding different isoforms. May play a role in prolonged long term-potentiation (LTP) maintenance. Contains 1 RING-type zinc finger.

Alternate Names

HZF, HZFWLTP (long-term potentiation) induced RING finger protein, LIRFHZFw1, Protein HZFw, ring finger protein 39

Gene Symbol

RNF39

Additional RNF39 Products

Product Documents for RNF39 Antibody (OTI5E10) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RNF39 Antibody (OTI5E10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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