RNF7 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-46159

Novus Biologicals, part of Bio-Techne
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NBP1-46159
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all RNF7 Antibodies »

Product Specifications

Immunogen

Peptide with sequence DACLRCQAENKQE corresponding to internal region according to NP_055060.1.

Specificity

This antibody is expected to recognize isoform 1 (NP_055060.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

13 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RNF7 Antibody

Western Blot: RNF7 Antibody [NBP1-46159]

Western Blot: RNF7 Antibody [NBP1-46159]

Western Blot: RNF7 Antibody [NBP1-46159] - Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: RNF7 Antibody [NBP1-46159]

Immunohistochemistry-Paraffin: RNF7 Antibody [NBP1-46159]

Immunohistochemistry-Paraffin: RNF7 Antibody [NBP1-46159] - Staining of Human Kidney. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for RNF7 Antibody

Application
Recommended Usage

Immunohistochemistry

5 ug/mL

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:8000

Western Blot

1 - 3 ug/mL
Application Notes
WB: Approx. 11 kDa band observed in human human heart lysates (calculated MW of 12.7 kDa band according to NP_055060.1). An additional band of 28 kDa band was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than WB.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RNF7

The protein encoded by this gene is a highly conserved ring finger protein. It is an essential subunit of SKP1-cullin/CDC53-F box protein ubiquitin ligases, which are a part of the protein degradation machinery important for cell cycle progression and signal transduction. This protein interacts with, and is a substrate of, casein kinase II (CSNK2A1/CKII). The phosphorylation of this protein by CSNK2A1 has been shown to promote the degradation of IkappaBalpha (CHUK/IKK-alpha/IKBKA) and p27Kip1(CDKN1B). Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Alternate Names

CKBBP1zinc RING finger protein SAG, CKII beta-binding protein 1, Rbx2, Regulator of cullins 2, ring finger protein 7rbx2, ROC2RING-box protein 2, SAGsensitive to apoptosis, zinc RING finger protein SAG, regulator of cullins 2, Sensitive to apoptosis gene protein

Entrez Gene IDs

9616 (Human); 19823 (Mouse)

Gene Symbol

RNF7

UniProt

Q9UBF6 (Human)

Additional RNF7 Products

Product Documents for RNF7 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RNF7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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