RNGTT Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83281

Novus Biologicals, part of Bio-Techne
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NBP1-83281
NBP1-83281-25ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RNGTT Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IKLLDLKPYKVSWKADGTRYMMLIDGTNEVFMIDRDNSVFHVSNLEFPFRKDLRMHLSNTLLDGEMIIDRVNGQAVPRYLIYDIIKFNSQPVGDCDFNVRLQCIEREIISPRHEKMKTGLIDKTQEP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RNGTT Antibody

Western Blot: RNGTT Antibody [NBP1-83281]

Western Blot: RNGTT Antibody [NBP1-83281]

Western Blot: RNGTT Antibody [NBP1-83281] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: RNGTT Antibody [NBP1-83281]

Immunocytochemistry/ Immunofluorescence: RNGTT Antibody [NBP1-83281]

Immunocytochemistry/Immunofluorescence: RNGTT Antibody [NBP1-83281] - Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: RNGTT Antibody [NBP1-83281]

Immunohistochemistry-Paraffin: RNGTT Antibody [NBP1-83281]

Immunohistochemistry-Paraffin: RNGTT Antibody [NBP1-83281] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells and decidual cells.
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Applications for RNGTT Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RNGTT

Bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus

Alternate Names

CAP1AmRNA-capping enzyme, hCAP, HCE1DKFZp686J2031, HCEHCAP1, RNA guanylyltransferase and 5'-phosphatase

Gene Symbol

RNGTT

Additional RNGTT Products

Product Documents for RNGTT Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RNGTT Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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