ROBLD3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-71687

Novus Biologicals, part of Bio-Techne
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NBP1-71687
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all ROBLD3 Antibodies »

Product Summary for ROBLD3 Antibody

Immunogen

A genomic peptide made to an N-terminal region of the human ROBLD3 protein (within residues 1-125). [Swiss-Prot Q9Y2Q5]

Localization

Late endosome membrane and Lysosome membrane.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ROBLD3 Antibody

Immunocytochemistry/ Immunofluorescence: ROBLD3 Antibody [NBP1-71687]

Immunocytochemistry/ Immunofluorescence: ROBLD3 Antibody [NBP1-71687]

Immunocytochemistry/Immunofluorescence: ROBLD3 Antibody [NBP1-71687] - ROBLD3 Antibody was tested at 1:100 in NIH/3T3 cells with FITC (green). Nuclei (Blue) were counterstained with DAPI (blue).
Immunohistochemistry: ROBLD3 Antibody [NBP1-71687]

Immunohistochemistry: ROBLD3 Antibody [NBP1-71687]

Immunohistochemistry: ROBLD3 Antibody [NBP1-71687] - Analysis of ROBLD3 in human renal cell carcinoma.

Applications for ROBLD3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry

1:100-1:200

Immunohistochemistry-Paraffin

1:100-1:200
Application Notes
This ROBLD3 antibody is useful for IHC-P and ICC/IF. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 0.1% BSA, and 50% Glycerol

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ROBLD3

ROBDL3/p14 is a major component of the Ragulator complex, which interacts with Rag GTPases and partially activates mTORC1 kinase. This interaction ultimately leads to growth, with influence of growth factors, energy levels, and amino acids. ROBDL3 is intrinsically related to lysosomal function, and endosomal transport regulation. It binds Nbr1 and the two are associated with protein turnover regulation, while it is required to localize MEK1 partner protein to the endosome. Endosomal localization of ROBLD3 can be affected by the interaction of p18.

Alternate Names

ENDAP, Endosomal adaptor protein p14, HSPC003, Late endosomal/lysosomal adaptor and MAPK and MTOR activator 2, late endosomal/lysosomal adaptor, MAPK and MTOR activator 2, Late endosomal/lysosomal Mp1-interacting protein, MAPBPIPRoadblock domain-containing protein 3, MAPKSP1 adaptor protein, MAPKSP1AP, Mitogen-activated protein-binding protein-interacting protein, mitogen-activated protein-binding protein-interacting protein (MAPBPIP), p14Ragulator2, roadblock domain containing 3, ROBLD3

Entrez Gene IDs

28956 (Human); 56692 (Mouse)

Gene Symbol

LAMTOR2

UniProt

Q9Y2Q5 (Human), O88653 (Mouse)

Additional ROBLD3 Products

Product Documents for ROBLD3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ROBLD3 Antibody

Manufactured by Genomic Antibody Technology™. GAT FAQs

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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