Immunocytochemistry/Immunofluorescence: Robo4 Antibody [NB110-58778] - Robo4 antibody was tested in Huvec cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunohistochemistry: ROBO4 Antibody [NB110-58778]

Immunohistochemistry: Robo4 Antibody [NB110-58778] - Detection of ROBO4 in endothelium and trophoblasts of the placenta.

Applications for ROBO4 Antibody

Application

Recommended Usage

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1:500

Application Notes

This Robo4 antibody is useful for Immunohistochemistry-Paraffin and Western Blot analysis, where bands at 85 kDa and 107 kDa are observed representing different isoforms of ROBO4 protein.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.1% Sodium Azide

Concentration

1.09 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ROBO4

Robo4 (roundabout homolog 4 or magic roundabout) is a receptor for Slit proteins which implicate in angiogenesis and vascular patterning especially through facilitating inhibition of primary endothelial cell migration. It is a fairly recently identified member of Robo family and is shorter than other Robo members at both intracellular as well as extracellular domains. Robo4 plays important roles in several aspects of vascular development such as the guidance of endothelial cell migration, cell cycle regulation, inhibition of pathologic angiogenesis and endothelial hyperpermeability etc. Employing mouse and zebrafish models, Slit2/Robo4 interactions have been shown to maintain integrity of vascular network and its barrier function by inhibiting cytokine-mediated vasculogenesis with enhanced permeability, and that Slit2-Robo4-paxillin-GIT1 network inhibits neovascularization and vascular leakage. Moreover, single nucleotide polymorphorism of Robo4 has been documented to be associated with autism suggesting its role in development and function of human brain.

Long Name

Roundabout Homolog 4

Alternate Names

ECSM4, FLJ20798, Magic roundabout, MGC133352, MGC133353, MRB, roundabout homolog 4, roundabout homolog 4, magic roundabout (Drosophila)

Gene Symbol

ROBO4

Additional ROBO4 Products

Product Documents for ROBO4 Antibody

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download ICC/IF Protocol

Download Protocol

COA

SDS

Download SDS

Product Specific Notices for ROBO4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

ROBO4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB110-58778

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

Concentration

View all ROBO4 Antibodies »

Product Specifications

Immunogen

Localization

Specificity

Marker

Clonality

Host

Isotype

Scientific Data Images for ROBO4 Antibody

Western Blot: ROBO4 Antibody [NB110-58778]

Immunocytochemistry/ Immunofluorescence: ROBO4 Antibody [NB110-58778]

Immunohistochemistry: ROBO4 Antibody [NB110-58778]

Applications for ROBO4 Antibody

Immunohistochemistry

Immunohistochemistry-Paraffin

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: ROBO4

Long Name

Alternate Names

Gene Symbol

Additional ROBO4 Products

Product Documents for ROBO4 Antibody

Product Datasheets

Protocols & Manuals

COA

Certificate of Analysis

SDS

Product Specific Notices for ROBO4 Antibody