ROBO4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB110-58780

Novus Biologicals, part of Bio-Techne
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NB110-58780
NB110-58780SS
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all ROBO4 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a C-terminal region of human Robo4 (within residues 900-1007). [Swiss-Prot# Q8WZ75]

Localization

Cell Membrane , cytoplasmic vesicles.

Specificity

This recognizes the 85 kDa and 107 kDa isoforms of the ROBO4 human protein.

Marker

Tumor Endothelial Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ROBO4 Antibody

Western Blot: ROBO4 Antibody [NB110-58780]

Western Blot: ROBO4 Antibody [NB110-58780]

Western Blot: Robo4 Antibody [NB110-58780] - Detection of ROBO4 in HUVEC lysate.
Immunocytochemistry/ Immunofluorescence: ROBO4 Antibody [NB110-58780]

Immunocytochemistry/ Immunofluorescence: ROBO4 Antibody [NB110-58780]

Immunocytochemistry/Immunofluorescence: Robo4 Antibody [NB110-58780] - Robo4 antibody was tested in Huvec cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry: ROBO4 Antibody [NB110-58780]

Immunohistochemistry: ROBO4 Antibody [NB110-58780]

Immunohistochemistry: Robo4 Antibody [NB110-58780] - Detection of ROBO4 in human placental epithelial cells and trophoblasts.

Applications for ROBO4 Antibody

Application
Recommended Usage

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1:500
Application Notes
This Robo4 antibody is useful for Immunohistochemistry-Paraffin and Western Blot analysis, where bands at 85 kDa and 107 kDa are observed representing different isoforms of ROBO4 protein.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ROBO4

Robo4 (roundabout homolog 4 or magic roundabout) is a receptor for Slit proteins which implicate in angiogenesis and vascular patterning especially through facilitating inhibition of primary endothelial cell migration. It is a fairly recently identified member of Robo family and is shorter than other Robo members at both intracellular as well as extracellular domains. Robo4 plays important roles in several aspects of vascular development such as the guidance of endothelial cell migration, cell cycle regulation, inhibition of pathologic angiogenesis and endothelial hyperpermeability etc. Employing mouse and zebrafish models, Slit2/Robo4 interactions have been shown to maintain integrity of vascular network and its barrier function by inhibiting cytokine-mediated vasculogenesis with enhanced permeability, and that Slit2-Robo4-paxillin-GIT1 network inhibits neovascularization and vascular leakage. Moreover, single nucleotide polymorphorism of Robo4 has been documented to be associated with autism suggesting its role in development and function of human brain.

Long Name

Roundabout Homolog 4

Alternate Names

ECSM4, FLJ20798, Magic roundabout, MGC133352, MGC133353, MRB, roundabout homolog 4, roundabout homolog 4, magic roundabout (Drosophila)

Entrez Gene IDs

54538 (Human)

Gene Symbol

ROBO4

UniProt

Q8WZ75

Additional ROBO4 Products

Product Documents for ROBO4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ROBO4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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