ROCK1 Antibody (12M03)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-46006

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # 12M03

Concentration

0.5 mg/ml

View all ROCK1 Antibodies »

Product Summary for ROCK1 Antibody (12M03)

Immunogen

Synthetic peptide corresponding to the caspase-3 cleavage site.

Reactivity Notes

Human.

Specificity

The antibody has been selected for its ability to recognize the cleaved ROCK-1 in Western blotting. It does not recognize the full length protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Scientific Data Images for ROCK1 Antibody (12M03)

Western Blot: ROCK1 Antibody (12M03) [NBP1-46006]

Western Blot: ROCK1 Antibody (12M03) [NBP1-46006]

Western Blot: cleaved ROCK1 Antibody (12M03) [NBP1-46006] - Jurkat T cells (Lane A:10 ug, Lane B: 25 ug, Lane C: 50 ug) stained with cleaved ROCK1 antibody.

Applications for ROCK1 Antibody (12M03)

Application
Recommended Usage

Western Blot

1 - 4 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2), 1% BSA, 50% Glycerol

Preservative

0.02% Thimerosal

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ROCK1

The Rho target protein, Rho-associated coiled coil-containing protein kinase (ROCK)-1 has been found to be a new Caspase-3 substrate. ROCK, one of the effectors of the small GTPase Rho, has recently been shown to contribute significantly to myosin light chain (MLC) activation through two pathways: direct phosphorylation of MLC and phosphorylation of MLC phosphatase, leading to its inhibition. The increase in cellular contractility that is necessary for apoptotic membrane blebbing implies sustained augmentation of MLC phosphorylation. ROCK-1 consists of an amino-terminal kinase domain and an inhibitory cysteine/histidine-rich C-terminal domain that is located within a pleckstrin-homology region. They are joined by a variable region that contains the Rho-binding domain. During apoptosis, ROCK-I is cleaved by caspase-3 at a conserved DETD1113/G sequence and its carboxy-terminal inhibitory domain is removed, resulting in deregulated and constitutive kinase activity. The caspase-3-mediated cleavage and activation of ROCK I induces phosphorylation of MLC and membrane blebbing, one of the first events in the execution phase of apoptosis.

Long Name

Rho-associated, Coiled-Coil Containing Protein Kinase 1

Alternate Names

p160ROCK, ROK beta

Entrez Gene IDs

6093 (Human)

Gene Symbol

ROCK1

UniProt

Q13464

Additional ROCK1 Products

Product Documents for ROCK1 Antibody (12M03)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ROCK1 Antibody (12M03)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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