ROCK1 Antibody (1H4) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37533

Novus Biologicals, part of Bio-Techne
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NBP2-37533
NBP2-37533-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1H4

Format

BSA Free

Concentration

1 mg/ml

View all ROCK1 Antibodies »

Product Summary for ROCK1 Antibody (1H4) - BSA Free

Immunogen

Purified recombinant fragment of human ROCK1 expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

158 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ROCK1 Antibody (1H4) - BSA Free

Western Blot: ROCK1 Antibody (1H4)BSA Free [NBP2-37533]

Western Blot: ROCK1 Antibody (1H4)BSA Free [NBP2-37533]

Western Blot: ROCK1 Antibody (1H4) [NBP2-37533] - Western blot analysis using ROCK1 mAb against HEK293 (1) and ROCK1 (AA: 403-610)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: ROCK1 Antibody (1H4)BSA Free [NBP2-37533]

Western Blot: ROCK1 Antibody (1H4)BSA Free [NBP2-37533]

Western Blot: ROCK1 Antibody (1H4) [NBP2-37533] - Western blot analysis using ROCK1 mAb against human ROCK1 (AA: 403-610) recombinant protein. (Expected MW is 158 kDa)
ELISA: ROCK1 Antibody (1H4) - BSA Free [NBP2-37533]

ELISA: ROCK1 Antibody (1H4) - BSA Free [NBP2-37533]

ELISA: ROCK1 Antibody (1H4) [NBP2-37533] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for ROCK1 Antibody (1H4) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ROCK1

Rho-associated kinase I (ROCK1, ROK beta, p160ROCK) is a serine-threonine protein kinase and an effector of the small GTPase Rho. With N-terminus protein kinase domain and C-terminus Rho-binding domain/pleckstrin homology domain, ROCK1 can be activated by either RhoA or RhoB. In Rho specific ROCK1 activation, a Rho protein binds to the Rho-binding domain and induces a conformational change which opens the kinase domain for the phosphorylation of downstream effectors (1). Activated ROCK1 phosphorylates various signaling proteins, such as myosin light chain phosphatase, LIM kinases, and ezrin-radixin-moesin proteins. Caspase-3 also activates ROCK1; Caspase-3 cleaves ROCK1 at DETD1113/G sequence and removes its inhibitory c-terminal domain. This activation, independent of Rho activity, leads to apoptotic membrane blebbing (2). ROCK1 is involved in regulating actin cytoskeleton assembly, cell migration, centromere positing, smooth muscle contraction, and neurite outgrowth. Its involvement in tumor invasion, hypertension, and bronchial asthma makes ROCK1 an ideal target for drug development (3).

Long Name

Rho-associated, Coiled-Coil Containing Protein Kinase 1

Alternate Names

p160ROCK, ROK beta

Gene Symbol

ROCK1

Additional ROCK1 Products

Product Documents for ROCK1 Antibody (1H4) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ROCK1 Antibody (1H4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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