Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Rabbit (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all ROCK1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1300 and the C-terminus (residue 1354) of human Rho-associated, coiled-coil containing protein kinase 1 using the numbering given in Swiss-Prot entry Q13464 (GeneID 6093).

Specificity

NB100-624 is specific for human Rho Kinase Beta protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ROCK1 Antibody

Western Blot: ROCK1 Antibody [NB100-624]

Western Blot: ROCK1 Antibody [NB100-624]

Western Blot: ROCK1 Antibody [NB100-624] - Detection of human and mouse ROCK1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ROCK1 antibody NB100-624 (lot NB100-624-2) used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. ROCK1 was also immunoprecipitated by a previous lot (lot NB100-624-1) of this antibody. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 1 second (B).

Applications for ROCK1 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:5000-1:15000
Application Notes
Use in IHC, IHC-P and ICC/IF reported in scientific literature (PMID 24324133).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using NB100-624 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ROCK1

Rho Kinase Beta, a 160 kDa small-GTP-effector protein, is a serine/threonine kinase involved in the induction of focal adhesions and stress fibers, and can play a major role in vascular smooth muscle contraction, malignant cell transformation, tumor invasion and metastasis. ROCK1 is identified as a substrate for caspases and is cleaved by caspase 3 at a conserved DETD1113/G sequence which removes its carboxy-terminal inhibitory domain, resulting in a constitutively active 130 kDa ROCK1 kinase. Proteolytic activation of ROCK1 by caspase is critical for its role in regulating the actin cytoskeleton and Myosin Light Chain (MLC) phosphorylation. Increased phosphorylation of MLC is important for membrane blebbing, a cellular event that occurs at the onset of apoptosis.

Long Name

Rho-associated, Coiled-Coil Containing Protein Kinase 1

Alternate Names

p160ROCK, ROK beta

Entrez Gene IDs

6093 (Human); 19877 (Mouse)

Gene Symbol

ROCK1

UniProt

Q13464

Additional ROCK1 Products

Product Documents for ROCK1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ROCK1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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