RPB2 Antibody (HL1042)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13697
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1042
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for RPB2 Antibody (HL1042)
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human RPB2. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Centrifuge briefly prior to opening.
Scientific Data Images for RPB2 Antibody (HL1042)
![Western Blot: RPB2 Antibody (HL1042) [NBP3-13697]](https://resources.bio-techne.com/images/products/RPB2-Antibody-HL1042-Western-Blot-NBP3-13697-img0001.jpg "Western Blot: RPB2 Antibody (HL1042) [NBP3-13697]")
Western Blot: RPB2 Antibody (HL1042) [NBP3-13697]
Western Blot: RPB2 Antibody (HL1042) [NBP3-13697] - 293T whole cell extracts (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1042] (NBP3-13697) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: RPB2 Antibody (HL1042) [NBP3-13697] -
Western Blot: RPB2 Antibody (HL1042) [NBP3-13697] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1042] (NBP3-13697) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry: RPB2 Antibody (HL1042) [NBP3-13697] -
Immunohistochemistry: RPB2 Antibody (HL1042) [NBP3-13697] - RPB2 antibody [HL1042] detects RPB2 protein on whole mount zebrafish by immunohistochemical analysis.Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.Green: RPB2 stained by RPB2 antibody [HL1042] (NBP3-13697) diluted at 1:100.Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC
Applications for RPB2 Antibody (HL1042)
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RPB2
Alternate Names
DNA-directed RNA polymerase II subunit RPB2, EC 2.7.7, EC 2.7.7.6, hRPB140, hsRPB2, POL2RB, polymerase (RNA) II (DNA directed) polypeptide B (140kD), polymerase (RNA) II (DNA directed) polypeptide B, 140kDa, RNA polymerase II subunit 2, RNA polymerase II subunit B2, RNA polymerase II subunit2, RNA polymerase II second largest subunit
Gene Symbol
POLR2B
Additional RPB2 Products
Product Documents for RPB2 Antibody (HL1042)
Product Specific Notices for RPB2 Antibody (HL1042)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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