RPL22 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-06069

Novus Biologicals, part of Bio-Techne
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NBP1-06069
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all RPL22 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-SKESYELRYFQINQ corresponding to C-Terminus according to NP_000974.1.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26962690).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for RPL22 Antibody

Western Blot: RPL22 Antibody [NBP1-06069]

Western Blot: RPL22 Antibody [NBP1-06069]

Western Blot: RPL22 Antibody [NBP1-06069] - hnRNP H/F subcellular localization and association with polysomal fractions. Polysome profile of U251 cells untreated (NT) or treated with 20 uM carboxypyridostatin (cPDS) for 1 h, and probing for hnRNP H/F or RPL22 (negative control), followed by western blot analysis from individual non-polysomal (NP) and polysomal (P) fractions by probing for hnRNP H/F, DHX36, DHX9, DDX3X, eIF4A. EEA1: negative control. RPS6: positive control. Shown is a representative result from n = 2 independent experiments. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41467-020-16168-x) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069]

Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069]

Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069] - Staining of paraffin embedded Human Cortex. Antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069]

Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069]

Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069] - Staining of paraffin embedded Human Thyroid. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Applications for RPL22 Antibody

Application
Recommended Usage

Immunohistochemistry

3 - 6 ug/mL

Immunohistochemistry-Paraffin

3-6 ug/ml

Peptide ELISA

detection limit 1:16000

Western Blot

1:100-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NBP1-06069 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RPL22

RPL22, also known as 60S ribosomal protein L22, is a 15kDa 128 amino acid protein. The primary function of RPL22 is to encode a cytoplasmic ribosomal protein. Current research is being conducted on RPL22 in relation to hepatitis, carcinoma, malaria, leukemia, hepatitis c, pneumonia and lung carcinoma. RPL22 has been linked to the virulence, methylation, contact inhibition, pathogenesis and cell growth pathways where it interacts with SHC1, UBA5, CSE1L, PRKAG1 and MAP3K14.

Alternate Names

EAPHBP15, EBER-associated protein, Epstein-Barr virus small RNA-associated protein, Epstein-Barr-encoded RNA-associated protein, HBP15/L22, heparin-binding protein 15, Heparin-binding protein HBp15, ribosomal protein L22,60S ribosomal protein L22

Entrez Gene IDs

6146 (Human); 19934 (Mouse); 81768 (Rat)

Gene Symbol

RPL22

UniProt

P35268 (Human)

Additional RPL22 Products

Product Documents for RPL22 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RPL22 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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