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RPL7 Antibody (2E10)

Novus Biologicals, part of Bio-Techne | Catalog # H00006129-M06

Novus Biologicals, part of Bio-Techne
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H00006129-M06

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2E10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

RPL7 (NP_000962.2, 158 a.a. ~ 248 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GYGKINKKRIALTDNALIARSLGKYGIICMEDLIHEIYTVGKRFKEANNFLWPFKLSSPRGGMKKKTTHFVEGGDAGNREDQINRLIRRMN

Specificity

RPL7 - ribosomal protein L7 (2E10)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for RPL7 Antibody (2E10)

Immunocytochemistry/ Immunofluorescence: RPL7 Antibody (2E10) [H00006129-M06]

Immunocytochemistry/ Immunofluorescence: RPL7 Antibody (2E10) [H00006129-M06]

Immunocytochemistry/Immunofluorescence: RPL7 Antibody (2E10) [H00006129-M06] - Analysis of monoclonal antibody to RPL7 on HeLa cell. Antibody concentration 10 ug/ml
ELISA: RPL7 Antibody (2E10) [H00006129-M06]

ELISA: RPL7 Antibody (2E10) [H00006129-M06]

ELISA: RPL7 Antibody (2E10) [H00006129-M06] - Detection limit for recombinant GST tagged RPL7 is approximately 3ng/ml as a capture antibody.

Applications for RPL7 Antibody (2E10)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
It has been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RPL7

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L30P family of ribosomal proteins. It contains an N-terminal basic region-leucine zipper (BZIP)-like domain and the RNP consensus submotif RNP2. In vitro the BZIP-like domain mediates homodimerization and stable binding to DNA and RNA, with a preference for 28S rRNA and mRNA. The protein can inhibit cell-free translation of mRNAs, suggesting that it plays a regulatory role in the translation apparatus. It is located in the cytoplasm. The protein has been shown to be an autoantigen in patients with systemic autoimmune diseases, such as systemic lupus erythematosus. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]

Alternate Names

humL7-1,60S ribosomal protein L7, MGC117326, ribosomal protein L7

Entrez Gene IDs

6129 (Human)

Gene Symbol

RPL7

Additional RPL7 Products

Product Documents for RPL7 Antibody (2E10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RPL7 Antibody (2E10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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