RPL7 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2268
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Predicted:
Bovine (100%), Chicken (100%), Primate (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunoprecipitation (Negative), Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 75 and 125 of human Ribosomal Protein 7 using the numbering given in entry NP_000962.2 (GeneID 6129).
Reactivity Notes
Orangutan (100%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for RPL7 Antibody
Western Blot: RPL7 Antibody [NB100-2268]
Western Blot: RPL7 Antibody [NB100-2268] - Staufen1 co-fractionates with Gag and vRNA in gradient density fractionation analyses. HeLa cells were either mock transfected with empty vector, pcDNA3, or with a plasmid that expresses Staufen1-HA. The transfected cells were collected 24?h later, lysed and were either mock-treated or treated with RNAse A. The lysates were then fractionated on 5-50% sucrose gradients and 20 fractions were collected for further analysis by western blotting for viral and host proteins, as indicated. HIV-1 viral genomic RNA (vRNA, 9?kb) was assessed in each fraction by slot blot analysis. TL represents the total lysates. Image collected and cropped by CiteAb from the following publication (https://www.frontiersin.org/Journal/10.3389/fmicb.2012.00367/full), licensed under a CC-BY license.Western Blot: RPL7 Antibody [NB100-2268]
Western Blot: RPL7 Antibody [NB100-2268] - Detection of Human and Mouse RPL7 on HeLa whole cell lysate using NB100-2268. RPL7 was immunoprecipitated by rabbit anti-RPL7 antibody NB100-2269.Western Blot: RPL7 Antibody [NB100-2268]
Western Blot: RPL7 Antibody [NB100-2268] - Staufen1 co-fractionates with Gag and vRNA in gradient density fractionation analyses. HeLa cells were co-transfected with pNL 4-3 and Staufen1-HA. The presence of Staufen1-HA, precursor Gag and p24 were assessed in each fraction by western blotting analysis. HIV-1 viral genomic RNA (9?kb, vRNA) was assessed in each fraction by slot blot analysis. Staufen1, Gag and vRNA were quantitated in each fraction by densitometry and relative levels are depicted for each fraction (Blue: Staufen1-HA, Red: Gag, Green-vRNA). Image collected and cropped by CiteAb from the following publication (https://www.frontiersin.org/Journal/10.3389/fmicb.2012.00367/full), licensed under a CC-BY license.Applications for RPL7 Antibody
Application
Recommended Usage
Western Blot
1:2000-1:10000
Application Notes
Immunoprecipitation: Not recommended. Use rabbit anti-RPL7 Antibody NB 100-2269.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: RPL7
Alternate Names
humL7-1,60S ribosomal protein L7, MGC117326, ribosomal protein L7
Gene Symbol
RPL7
UniProt
Additional RPL7 Products
Product Documents for RPL7 Antibody
Product Specific Notices for RPL7 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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