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RPLP2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15104

Novus Biologicals, part of Bio-Techne
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NBP2-15104

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (97%), Mouse (98%), Porcine (95%), Rat (97%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for RPLP2 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human RPLP2. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RPLP2 Antibody

Immunocytochemistry/ Immunofluorescence: RPLP2 Antibody [NBP2-15104]

Immunocytochemistry/ Immunofluorescence: RPLP2 Antibody [NBP2-15104]

Immunocytochemistry/Immunofluorescence: RPLP2 Antibody [NBP2-15104] - Analysis of methanol-fixed A431, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: RPLP2 Antibody [NBP2-15104]

Immunohistochemistry-Paraffin: RPLP2 Antibody [NBP2-15104]

Immunohistochemistry-Paraffin: RPLP2 Antibody [NBP2-15104] - Human colon carcinoma, using RPLP2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
RPLP2 Antibody

Western Blot: RPLP2 Antibody [NBP2-15104] -

Western Blot: RPLP2 Antibody [NBP2-15104] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with RPLP2 antibody (NBP2-15104) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for RPLP2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:5000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RPLP2

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal phosphoprotein that is a component of the 60S subunit. The protein, which is a functional equivalent of the E. coli L7/L12 ribosomal protein, belongs to the L12P family of ribosomal proteins. It plays an important role in the elongation step of protein synthesis. Unlike most ribosomal proteins, which are basic, the encoded protein is acidic. Its C-terminal end is nearly identical to the C-terminal ends of the ribosomal phosphoproteins P0 and P1. The P2 protein can interact with P0 and P1 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. The protein is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]

Alternate Names

acidic ribosomal phosphoprotein P2, MGC71408,60S acidic ribosomal protein P2, P2, Renal carcinoma antigen NY-REN-44, ribosomal protein, large, P2, RPP2D11S2243E

Gene Symbol

RPLP2

Additional RPLP2 Products

Product Documents for RPLP2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RPLP2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.