Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Drosophila (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all RPS14 Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 101 to 151 of human Ribosomal Protein S14 using the numbering given in entry NP_001020242.1 (GeneID 6208).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RPS14 Antibody

Western Blot: RPS14 Antibody [NBP2-22319]

Western Blot: RPS14 Antibody [NBP2-22319]

Western Blot: RPS14 Antibody [NBP2-22319] - Whole cell lysate (50 ug) from 293T, HeLa, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. NBP2-22319 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: RPS14 Antibody [NBP2-22319]

Immunoprecipitation: RPS14 Antibody [NBP2-22319]

Immunoprecipitation: RPS14 Antibody [NBP2-22319] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22319 used for IP at 6 ug/mg lysate. RPS14 was also immunoprecipitated by rabbit anti-RPS14 antibody. For blotting immunoprecipitated RPS14, NBP2-22319 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for RPS14 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:2000 - 1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-12% SDS-PAGE.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RPS14

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S11P family of ribosomal proteins. It is located in the cytoplasm. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. In Chinese hamster ovary cells, mutations in this gene can lead to resistance to emetine, a protein synthesis inhibitor. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

Alternate Names

emetine resistance, EMTB40S ribosomal protein S14, ribosomal protein S14

Gene Symbol

RPS14

UniProt

NP_001020242.1 (Human)

Additional RPS14 Products

Product Documents for RPS14 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RPS14 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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