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RPS3 Antibody (3X2M4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16496

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-16496-100ul
NBP3-16496-20ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3X2M4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human RPS3 (P23396). AQAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEVVVSGKLRGQRAKSMKFVDGLMIHSGDPVNYYVDTAVRHVLLRQGVLGIKVKIMLPWDPTGKIGP

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RPS3 Antibody (3X2M4)

Western Blot: RPS3 Antibody (3X2M4) [NBP3-16496]

Western Blot: RPS3 Antibody (3X2M4) [NBP3-16496]

Western Blot: RPS3 Antibody (3X2M4) [NBP3-16496] - Western blot analysis of extracts of various cell lines, using RPS3 Rabbit mAb (NBP3-16496) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: RPS3 Antibody (3X2M4) [NBP3-16496]

Immunocytochemistry/ Immunofluorescence: RPS3 Antibody (3X2M4) [NBP3-16496]

Immunocytochemistry/Immunofluorescence: RPS3 Antibody (3X2M4) [NBP3-16496] - Immunofluorescence analysis of NIH-3T3 cells using RPS3 Rabbit mAb (NBP3-16496) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for RPS3 Antibody (3X2M4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RPS3

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit, where it forms part of the domain where translation is initiated. The protein belongs to the S3P family of ribosomal proteins. Studies of the mouse and rat proteins have demonstrated that the protein has an extraribosomal role as an endonuclease involved in the repair of UV-induced DNA damage. The protein appears to be located in both the cytoplasm and nucleus but not in the nucleolus. Higher levels of expression of this gene in colon adenocarcinomas and adenomatous polyps compared to adjacent normal colonic mucosa have been observed. This gene is co-transcribed with the small nucleolar RNA genes U15A and U15B, which are located in its first and fifth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]

Alternate Names

40S ribosomal protein S3, FLJ26283, FLJ27450, IMR-90 ribosomal protein S3, MGC87870, ribosomal protein S3

Gene Symbol

RPS3

Additional RPS3 Products

Product Documents for RPS3 Antibody (3X2M4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RPS3 Antibody (3X2M4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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