Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # RP159-1

Format

Azide and BSA Free

Concentration

1 mg/ml

View all RPS3 Antibodies »

Product Summary for RPS3 Antibody (RP159-1) - Azide and BSA Free

Immunogen

Recombinant human RPS3.

Specificity

Recognizes human RPS3. Detects a band of ~32kDa by Western blot.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for RPS3 Antibody (RP159-1) - Azide and BSA Free

Western Blot: RPS3 Antibody (RP159-1)Azide and BSA Free [NBP2-80043]

Western Blot: RPS3 Antibody (RP159-1)Azide and BSA Free [NBP2-80043]

Western Blot: RPS3 Antibody (RP159-1) [NBP2-80043] - Western blot analysis using anti-Ribosomal Protein S3 (human), mAb (RP159-1) at 1:500 dilution.1: Human RPS3.2: HepG2 cell lysate (70 ug).3: HEK 293 cell lysate (70 ug).
Immunohistochemistry-Paraffin: RPS3 Antibody (RP159-1) - Azide and BSA Free [NBP2-80043]

Immunohistochemistry-Paraffin: RPS3 Antibody (RP159-1) - Azide and BSA Free [NBP2-80043]

Immunohistochemistry-Paraffin: RPS3 Antibody (RP159-1) [NBP2-80043] - Immunohistochemical staining of Ribosomal Protein S3 using anti-Ribosomal Protein S3 (human), mAb (RP159-1) in human kidney tissue (5 ug/mL). This antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

Applications for RPS3 Antibody (RP159-1) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:500 - 1:5000

Immunohistochemistry

5 ug/mL

Immunohistochemistry-Paraffin

5 ug/mL

Western Blot

1:500 - 1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um-filtered solution in PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RPS3

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit, where it forms part of the domain where translation is initiated. The protein belongs to the S3P family of ribosomal proteins. Studies of the mouse and rat proteins have demonstrated that the protein has an extraribosomal role as an endonuclease involved in the repair of UV-induced DNA damage. The protein appears to be located in both the cytoplasm and nucleus but not in the nucleolus. Higher levels of expression of this gene in colon adenocarcinomas and adenomatous polyps compared to adjacent normal colonic mucosa have been observed. This gene is co-transcribed with the small nucleolar RNA genes U15A and U15B, which are located in its first and fifth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]

Alternate Names

40S ribosomal protein S3, FLJ26283, FLJ27450, IMR-90 ribosomal protein S3, MGC87870, ribosomal protein S3

Gene Symbol

RPS3

Additional RPS3 Products

Product Documents for RPS3 Antibody (RP159-1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RPS3 Antibody (RP159-1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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