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RPS3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33691

Novus Biologicals, part of Bio-Techne
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NBP1-33691

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (99%), Chicken (98%), Chimpanzee (100%), Porcine (100%), Rhesus Macaque (100%), Xenopus (99%), Zebrafish (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human RPS3. The exact sequence is proprietary.

Reactivity Notes

Drosophila (86%), Xenopus laevis (99%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RPS3 Antibody

Immunocytochemistry/ Immunofluorescence: RPS3 Antibody [NBP1-33691]

Immunocytochemistry/ Immunofluorescence: RPS3 Antibody [NBP1-33691]

Immunocytochemistry/Immunofluorescence: RPS3 Antibody [NBP1-33691] - Analysis of methanol-fixed HeLa, using RPS3 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.
Immunohistochemistry-Paraffin: RPS3 Antibody [NBP1-33691]

Immunohistochemistry-Paraffin: RPS3 Antibody [NBP1-33691]

Immunohistochemistry-Paraffin: RPS3 Antibody [NBP1-33691] - Mouse intestine diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: RPS3 Antibody [NBP1-33691]

Immunohistochemistry-Paraffin: RPS3 Antibody [NBP1-33691]

Immunohistochemistry-Paraffin: RPS3 Antibody [NBP1-33691] - SAS xenograft, using antibody at 1:500 dilution. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min

Applications for RPS3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

Assay dependent

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RPS3

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit, where it forms part of the domain where translation is initiated. The protein belongs to the S3P family of ribosomal proteins. Studies of the mouse and rat proteins have demonstrated that the protein has an extraribosomal role as an endonuclease involved in the repair of UV-induced DNA damage. The protein appears to be located in both the cytoplasm and nucleus but not in the nucleolus. Higher levels of expression of this gene in colon adenocarcinomas and adenomatous polyps compared to adjacent normal colonic mucosa have been observed. This gene is co-transcribed with the small nucleolar RNA genes U15A and U15B, which are located in its first and fifth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]

Alternate Names

40S ribosomal protein S3, FLJ26283, FLJ27450, IMR-90 ribosomal protein S3, MGC87870, ribosomal protein S3

Gene Symbol

RPS3

Additional RPS3 Products

Product Documents for RPS3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RPS3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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