RRBP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83957

Novus Biologicals, part of Bio-Techne
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NBP1-83957
NBP1-83957-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RRBP1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ELHSKLQSSEAEVRSKCEELSGLHGQLQEARAENSQLTERIRSIEALLEAGQARDAQDVQASQAEADQQQTRLKELESQVSGLEKEAIELREAVEQQKVKNNDLREK

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RRBP1 Antibody

Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-83957]

Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-83957]

Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-83957] - Analysis in human placenta and skeletal muscle tissues. Corresponding RRBP1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-83957]

Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-83957]

Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-83957] - Staining of human colon, kidney, lymph node and placenta using Anti-RRBP1 antibody NBP1-83957 (A) shows similar protein distribution across tissues to independent antibody NBP1-83958 (B).
Western Blot: RRBP1 Antibody [NBP1-83957]

Western Blot: RRBP1 Antibody [NBP1-83957]

Western Blot: RRBP1 Antibody [NBP1-83957] - Analysis in human cell line CACO-2.
View 10 more images

Applications for RRBP1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Simple Western

1:200

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RRBP1

Analysis of cDNA clones indicates that ribosome binding protein 1 may exist in different forms due to removal of tandem repeats, or partial intraexonic splicing of RRBP1. The form presented here is lacking the canine p180 ribosome-binding domain, NQGKKAEGAQ, which is tandemly repeated close to the N-terminus in other forms that haven't been fully characterized. RRBP1 has been excluded as a candidate gene in the cause of Alagille syndrome. Alternate splicing results in multiple transcript variants. (provided by RefSeq)

Alternate Names

180 kDa ribosome receptor homolog, DKFZp586A1420, ES/130, ES/130-related protein, ES130, FLJ36146, hES, KIAA1398, MGC157720, MGC157721, ribosome binding protein 1 (dog 180kD homolog), ribosome binding protein 1 homolog 180kDa (dog), Ribosome receptor protein, ribosome-binding protein 1, RRp

Gene Symbol

RRBP1

Additional RRBP1 Products

Product Documents for RRBP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RRBP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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