RSK1 [p Thr359, p Ser363] Antibody (1B6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26394

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1B6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all RSK1 Antibodies »

Product Summary for RSK1 [p Thr359, p Ser363] Antibody (1B6)

Immunogen

A synthesized peptide derived from Human RSK1 [p Thr359, p Ser363] [UniProt Q15418]

Modification

p Thr 359, p Ser 363

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RSK1 [p Thr359, p Ser363] Antibody (1B6)

RSK1 [p Thr359, p Ser363] Antibody (1B6)

Immunoprecipitation: RSK1 [p Thr359, p Ser363] Antibody (1B6) [NBP3-26394] -

Immunoprecipitation: RSK1 [p Thr359, p Ser363] Antibody (1B6) [NBP3-26394] - Immunoprecipitating RSK1 [p Thr359, p Ser363] in Hela whole cell lysate.
Lane 1: Rabbit control IgG (1ug) instead of NBP3-26394 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26394 (3ug) + Hela whole cell lysate (1mg).
Lane 3: Hela whole cell lysate (20ug).
RSK1 [p Thr359, p Ser363] Antibody (1B6)

Western Blot: RSK1 [p Thr359, p Ser363] Antibody (1B6) [NBP3-26394] -

Western Blot: RSK1 [p Thr359, p Ser363] Antibody (1B6) [NBP3-26394] - Positive Western Blot detected in HepG2 whole cell lysate (treated with EGF or not).
All lanes: RSK1 [p Thr359, p Ser363] Antibody at 0.69ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 90 KDa
Observed band size: 90 KDa
RSK1 [p Thr359, p Ser363] Antibody (1B6)

Immunocytochemistry/Immunofluorescence: RSK1 [p Thr359, p Ser363] Antibody (1B6) [NBP3-26394] -

Immunocytochemistry/Immunofluorescence: RSK1 [p Thr359, p Ser363] Antibody (1B6) [NBP3-26394] - Staining of Hela cells with RSK1 [p Thr359, p Ser363] Antibody (1B6) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).

Applications for RSK1 [p Thr359, p Ser363] Antibody (1B6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:200

Immunoprecipitation

1:200-1:1000

Western Blot

1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: RSK1

Rsk-1 (p90-Rsk-1) is a member of a family of 90 kDa ribosomal protein S6 kinases, which includes Rsk-1, Rsk-2 and Rsk-3. These are broadly expressed serine/threonine protein kinases (1) activated in response to mitogenic stimuli, including extracellular signal-regulated protein kinases Erk1 and Erk2 (2). p90-Rsk-1 is activated by MAPK in vitro and in vivo via phosphorylation (3). Active Rsks appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB (2,4).

Long Name

Ribosomal Protein S6 Kinase I

Alternate Names

HU-1, MAPKAPL1A, RPS6KA1

Gene Symbol

RPS6KA1

Additional RSK1 Products

Product Documents for RSK1 [p Thr359, p Ser363] Antibody (1B6)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RSK1 [p Thr359, p Ser363] Antibody (1B6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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