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RTN4IP1 Antibody (OTI3B2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00478

Novus Biologicals, part of Bio-Techne
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NBP2-00478

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3B2

Concentration

0.38 mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human RTN4IP1 (NP_116119) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

38.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RTN4IP1 Antibody (OTI3B2)

Western Blot: RTN4IP1 Antibody (OTI3B2) [NBP2-00478]

Western Blot: RTN4IP1 Antibody (OTI3B2) [NBP2-00478]

Western Blot: RTN4IP1 Antibody (3B2) [NBP2-00478] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RTN4IP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RTN4IP1.
Immunohistochemistry-Paraffin: RTN4IP1 Antibody (3B2) [NBP2-00478] Staining of paraffin-embedded Human pancreas tissue using anti-RTN4IP1 mouse monoclonal antibody.
Flow Cytometry: RTN4IP1 Antibody (OTI3B2) [NBP2-00478]

Flow Cytometry: RTN4IP1 Antibody (OTI3B2) [NBP2-00478]

Flow Cytometry: RTN4IP1 Antibody (3B2) [NBP2-00478] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RTN4IP1 antibody, and then analyzed by flow cytometry.

Applications for RTN4IP1 Antibody (OTI3B2)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.38 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RTN4IP1

This gene encodes a novel mitochondrial protein that interacts with reticulon 4, which is a potent inhibitor of regeneration following spinal cord injury. The interaction of reticulon 4 with mitochondrial proteins may provide insight into the mechanisms for reticulon-induced inhibition of neurite growth. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.

Alternate Names

MGC12934, NIMPNOGO-interacting mitochondrial protein, reticulon 4 interacting protein 1, reticulon-4-interacting protein 1, mitochondrial

Entrez Gene IDs

84816 (Human)

Gene Symbol

RTN4IP1

Additional RTN4IP1 Products

Product Documents for RTN4IP1 Antibody (OTI3B2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RTN4IP1 Antibody (OTI3B2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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