RTN4IP1 Antibody (OTI3B2) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73952

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3B2

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all RTN4IP1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human RTN4IP1 (NP_116119) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

38.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RTN4IP1 Antibody (OTI3B2) - Azide and BSA Free

Western Blot: RTN4IP1 Antibody (OTI3B2)Azide and BSA Free [NBP2-73952]

Western Blot: RTN4IP1 Antibody (OTI3B2)Azide and BSA Free [NBP2-73952]

Western Blot: RTN4IP1 Antibody (OTI3B2) - Azide and BSA Free [NBP2-73952] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RTN4IP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RTN4IP1.
Immunohistochemistry: RTN4IP1 Antibody (OTI3B2) - Azide and BSA Free [NBP2-73952]

Immunohistochemistry: RTN4IP1 Antibody (OTI3B2) - Azide and BSA Free [NBP2-73952]

Immunohistochemistry: RTN4IP1 Antibody (OTI3B2) - Azide and BSA Free [NBP2-73952] - Staining of paraffin-embedded Human lymphoma tissue using anti-RTN4IP1 mouse monoclonal antibody.
Flow Cytometry: RTN4IP1 Antibody (OTI3B2) - Azide and BSA Free [NBP2-73952]

Flow Cytometry: RTN4IP1 Antibody (OTI3B2) - Azide and BSA Free [NBP2-73952]

Flow Cytometry: RTN4IP1 Antibody (OTI3B2) - Azide and BSA Free [NBP2-73952] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-RTN4IP1 antibody, and then analyzed by flow cytometry.
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Applications for RTN4IP1 Antibody (OTI3B2) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RTN4IP1

RTN4IP1 encodes a novel mitochondrial protein that interacts with reticulon 4, which is a potent inhibitor of regeneration following spinal cord injury. The interaction of reticulon 4 with mitochondrial proteins may provide insight into the mechanisms for reticulon-induced inhibition of neurite growth. [provided by RefSeq]

Alternate Names

MGC12934, NIMPNOGO-interacting mitochondrial protein, reticulon 4 interacting protein 1, reticulon-4-interacting protein 1, mitochondrial

Gene Symbol

RTN4IP1

Additional RTN4IP1 Products

Product Documents for RTN4IP1 Antibody (OTI3B2) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RTN4IP1 Antibody (OTI3B2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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