Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1H1

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all RUNX1T1/ETO Antibodies »

Product Summary for RUNX1T1/ETO Antibody (OTI1H1) - Azide and BSA Free

Immunogen

Full length human recombinant protein of human RUNX1T1(NP_783553) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RUNX1T1/ETO Antibody (OTI1H1) - Azide and BSA Free

Western Blot: RUNX1T1/ETO Antibody (OTI1H1)Azide and BSA Free [NBP2-73956]

Western Blot: RUNX1T1/ETO Antibody (OTI1H1)Azide and BSA Free [NBP2-73956]

Western Blot: RUNX1T1/ETO Antibody (OTI1H1) - Azide and BSA Free [NBP2-73956] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-RUNX1T1 monoclonal antibody (1:200).
Immunocytochemistry/ Immunofluorescence: RUNX1T1/ETO Antibody (OTI1H1) - Azide and BSA Free [NBP2-73956]

Immunocytochemistry/ Immunofluorescence: RUNX1T1/ETO Antibody (OTI1H1) - Azide and BSA Free [NBP2-73956]

Immunocytochemistry/Immunofluorescence: RUNX1T1/ETO Antibody (OTI1H1) - Azide and BSA Free [NBP2-73956] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RUNX1T1.
Western Blot: RUNX1T1/ETO Antibody (OTI1H1)Azide and BSA Free [NBP2-73956]

Western Blot: RUNX1T1/ETO Antibody (OTI1H1)Azide and BSA Free [NBP2-73956]

Western Blot: RUNX1T1/ETO Antibody (OTI1H1) - Azide and BSA Free [NBP2-73956] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RUNX1T1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RUNX1T1.
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Applications for RUNX1T1/ETO Antibody (OTI1H1) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RUNX1T1/ETO

RUNX1T1 / ETO is encoded by this gene is a putative zinc finger transcription factor and oncoprotein. In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Several transcript variants encoding multiple isoforms have been found for this gene.

Alternate Names

AML1T1protein CBFA2T1, CBFA2T1Cyclin-D-related protein, CDRMGC2796, core-binding factor, runt domain, alpha subunit 2; translocated to, 1; cyclinD-related, DKFZp564B213, Eight twenty one protein, ETOFLJ33145, MTG8acute myelogenous leukemia 1 translocation 1, cyclin-D related, Protein ETO, Protein MTG8, runt-related transcription factor 1; translocated to, 1 (cyclin D-related), Zinc finger MYND domain-containing protein 2, ZMYND2myeloid translocation gene on 8q22

Gene Symbol

RUNX1T1

Additional RUNX1T1/ETO Products

Product Documents for RUNX1T1/ETO Antibody (OTI1H1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RUNX1T1/ETO Antibody (OTI1H1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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