RUNX3/CBFA3 Antibody (2B10E8) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22391

Novus Biologicals, part of Bio-Techne
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NBP2-22391
NBP2-22391SS
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2B10E8

Format

BSA Free

Concentration

1.0 mg/ml

View all RUNX3/CBFA3 Antibodies »

Product Summary for RUNX3/CBFA3 Antibody (2B10E8) - BSA Free

Immunogen

Amino acids 126-310 of human Runx3 were used as the immunogen for this antibody.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for RUNX3/CBFA3 Antibody (2B10E8) - BSA Free

Western Blot: RUNX3/CBFA3 Antibody (2B10E8)BSA Free [NBP2-22391]

Western Blot: RUNX3/CBFA3 Antibody (2B10E8)BSA Free [NBP2-22391]

Western Blot: RUNX3/CBFA3 Antibody (2B10E8) [NBP2-22391] - Analysis of a partial recombinant Runx3 protein probed with Runx3 antibody at 0.5 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunohistochemistry-Paraffin: RUNX3/CBFA3 Antibody (2B10E8) - BSA Free [NBP2-22391]

Immunohistochemistry-Paraffin: RUNX3/CBFA3 Antibody (2B10E8) - BSA Free [NBP2-22391]

Immunohistochemistry-Paraffin: RUNX3/CBFA3 Antibody (2B10E8) [NBP2-22391] - Staining of normal human spleen tissue stained with Runx3 antibody (3 ug/ml), peroxidase-conjugate and DAB chromogen.
Flow Cytometry: RUNX3/CBFA3 Antibody (2B10E8) - BSA Free [NBP2-22391]

Flow Cytometry: RUNX3/CBFA3 Antibody (2B10E8) - BSA Free [NBP2-22391]

Flow Cytometry: RUNX3/CBFA3 Antibody (2B10E8) [NBP2-22391] - Intracellular flow analysis of human lymphocytes probed with Runx3 antibody (red) and isotype control (green) at 0.25 ug/10^6 cells.

Applications for RUNX3/CBFA3 Antibody (2B10E8) - BSA Free

Application
Recommended Usage

Flow Cytometry

0.25-1 ug/10^6 cells

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

3-5 ug/ml

Western Blot

0.5-2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RUNX3/CBFA3

RUNX3 binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters. RUNX3 consists of a heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer through the Runt domain. DNA-binding is increased by heterodimerization.

Long Name

Runt-related Transcription Factor 3

Alternate Names

AML2, CBFA3, PEBP2A3, PEBP2AC

Gene Symbol

RUNX3

UniProt

NP_001026850.1 (Human)

Additional RUNX3/CBFA3 Products

Product Documents for RUNX3/CBFA3 Antibody (2B10E8) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RUNX3/CBFA3 Antibody (2B10E8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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