RUNX3/CBFA3 Antibody (2B9G4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22390

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2B9G4

Concentration

0.5 mg/ml

View all RUNX3/CBFA3 Antibodies »

Product Summary for RUNX3/CBFA3 Antibody (2B9G4)

Immunogen

Amino acids 126-310 of human Runx3 were used as the immunogen for this antibody.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for RUNX3/CBFA3 Antibody (2B9G4)

Western Blot: RUNX3/CBFA3 Antibody (2B9G4) [NBP2-22390]

Western Blot: RUNX3/CBFA3 Antibody (2B9G4) [NBP2-22390]

Western Blot: RUNX3/CBFA3 Antibody (2B9G4) [NBP2-22390] - Analysis of a partial recombinant Runx3 protein probed with Runx3 antibody at 0.5 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunohistochemistry-Paraffin: RUNX3/CBFA3 Antibody (2B9G4) [NBP2-22390]

Immunohistochemistry-Paraffin: RUNX3/CBFA3 Antibody (2B9G4) [NBP2-22390]

Immunohistochemistry-Paraffin: RUNX3/CBFA3 Antibody (2B9G4) [NBP2-22390] - Normal human spleen (left) and colon cancer tissue (right) stained with RUNX3 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen.

Applications for RUNX3/CBFA3 Antibody (2B9G4)

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

5-7 ug/ml

Western Blot

2-5 ug/ml
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 ml PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RUNX3/CBFA3

RUNX3 binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters. RUNX3 consists of a heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer through the Runt domain. DNA-binding is increased by heterodimerization.

Long Name

Runt-related Transcription Factor 3

Alternate Names

AML2, CBFA3, PEBP2A3, PEBP2AC

Gene Symbol

RUNX3

UniProt

NP_001026850.1 (Human)

Additional RUNX3/CBFA3 Products

Product Documents for RUNX3/CBFA3 Antibody (2B9G4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RUNX3/CBFA3 Antibody (2B9G4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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