Ryanodine Receptor 2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90091

Novus Biologicals, part of Bio-Techne
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NBP1-90091
NBP1-90091-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Rat (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry-Paraffin

Cited:

Immunohistochemistry Free-Floating

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Ryanodine Receptor 2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QDEVRGDGEEGERKPLEAALPSEDLTDLKELTEESDLLSDIFGLDLKREGGQYKLIPHNPNAGLSDLMSNPVPMPEVQEKFQEQKAKEEEKEEKEETKSEPE

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30483051).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Ryanodine Receptor 2 Antibody

Immunohistochemistry-Paraffin: Ryanodine Receptor 2 Antibody [NBP1-90091]

Immunohistochemistry-Paraffin: Ryanodine Receptor 2 Antibody [NBP1-90091]

Immunohistochemistry-Paraffin: Ryanodine Receptor 2 Antibody [NBP1-90091] - Analysis in human heart muscle and liver tissues. Corresponding RYR2 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: Ryanodine Receptor 2 Antibody [NBP1-90091]

Immunocytochemistry/ Immunofluorescence: Ryanodine Receptor 2 Antibody [NBP1-90091]

Immunocytochemistry/Immunofluorescence: Ryanodine Receptor 2 Antibody [NBP1-90091] - Validation of enriched RYR2 expression in Fezf2+ve IT-PNs by immunohistochemistry. Coronal sections from CTB647 injected Fezf2-Gfp mouse were labeled for Ryanodine receptor 2 (RYR2) expression and images captured in M1. (i) RYR2 expression in M1. (ii) Image shows co-expression of RYR2 (purple) with Fezf2+ve IT-PNs (green and red; arrowhead) and Fezf2-ve IT-PNs (red; asterisk). Dashed lines indicate layer 5 from which cells were selected for quantification. Scale bar is 100 um. Image collected and cropped by CiteAb from the following publication (https://www.frontiersin.org/article/10.3389/fnmol.2018.00410/full), licensed under a CC-BY license.
Immunohistochemistry-Paraffin: Ryanodine Receptor 2 Antibody [NBP1-90091]

Immunohistochemistry-Paraffin: Ryanodine Receptor 2 Antibody [NBP1-90091]

Immunohistochemistry-Paraffin: Ryanodine Receptor 2 Antibody [NBP1-90091] - Staining of human heart muscle shows moderate to strong cytoplasmic positivity in cardiomyocytes.
View 3 more images

Applications for Ryanodine Receptor 2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry Free-Floating

Reported in scientific literature (PMID:30483051).

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ryanodine Receptor 2

Dihydropyridine receptor (DHPR) is a surface membrane protein critical for the excitation-contraction coupling of striated muscle. DHPR and the sarcoplasmic reticulum ryanodine receptor (RyR) are two key components of the intracellular junctions, where depolarization of the surface membrane is converted into the release of Ca2+ from internal stores. The alpha1-subunit of the DHPR contains a cytoplasmic loop which is thought to be involved in the interactions with RyR. Phosphorylation of the DHPR alpha1-subunit is also thought to play a role in the functional interaction of DHPR and RyR. Mutation in DHPR alpha1 results in excitation-contraction uncoupling, leading to muscular dysgenesis, a complete inactivity in developing skeletal muscles. Cells that do not express RyR also lack excitation-contraction coupling and exhibit a severalfold reduction in Ca2+ current density.

Long Name

Ryanodine receptor 2

Alternate Names

hRYR-2, RYR-2, RyR2

Gene Symbol

RYR2

Additional Ryanodine Receptor 2 Products

Product Documents for Ryanodine Receptor 2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Ryanodine Receptor 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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