RYBP/DEDAF Antibody (1F4)
Novus Biologicals, part of Bio-Techne | Catalog # H00023429-M08
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunoprecipitation, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 1F4
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
RYBP (AAH36459, 1 a.a. ~ 228 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTMGDKKSPTRPKRQAKPATDEGFWDCSVCTFRNSAEAFKCSICDVRKGTSTRKPRINSQLVAQQVAQQYATPPPPKKEKKEKVEKQDKEKPEKDKEISPSVTKKNTNKKTKPKSDILKDPPSEANSIQSANATTKTSETNHTSRPRLKNVDRSTAQQLAVTVGNVTVIITDFKEKTRSSSTSSSTVTSSAGSEQQNQSSSGSESTDKGSSRSSTPKGDMSAVNDESF
Specificity
RYBP - RING1 and YY1 binding protein (1F4)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for RYBP/DEDAF Antibody (1F4)
Western Blot: RYBP/DEDAF Antibody (1F4) [H00023429-M08]
Western Blot: RYBP/DEDAF Antibody (1F4) [H00023429-M08] - Analysis of RYBP expression in transfected 293T cell line by RYBP monoclonal antibody (M08), clone 1F4. Lane 1: RYBP transfected lysatE (24.8 KDa). Lane 2: Non-transfected lysate.Immunoprecipitation: RYBP/DEDAF Antibody (1F4) [H00023429-M08]
Immunoprecipitation: RYBP/DEDAF Antibody (1F4) [H00023429-M08] - Analysis of RYBP transfected lysate using anti-RYBP monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RYBP rabbit polyclonal antibody.
Sandwich ELISA: RYBP/DEDAF Antibody (1F4) [H00023429-M08] - Detection limit for recombinant GST tagged RYBP is 0.3 ng/ml as a capture antibody.
Applications for RYBP/DEDAF Antibody (1F4)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: RYBP/DEDAF
Alternate Names
AAP1, APAP-1, Apoptin-associating protein 1, Death effector domain-associated factor, DEDAFYY1 and E4TF1-associated factor 1, RING1 and YY1 binding protein, RING1 and YY1-binding protein, ring1 interactor RYBP, YEAF1DED-associated factor, YY1 and E4TF1 associated factor 1
Entrez Gene IDs
23429 (Human)
Gene Symbol
RYBP
UniProt
Additional RYBP/DEDAF Products
Product Documents for RYBP/DEDAF Antibody (1F4)
Product Specific Notices for RYBP/DEDAF Antibody (1F4)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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