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RYBP/DEDAF Antibody (1F4)

Novus Biologicals, part of Bio-Techne | Catalog # H00023429-M08

Novus Biologicals, part of Bio-Techne
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H00023429-M08

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1F4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

RYBP (AAH36459, 1 a.a. ~ 228 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTMGDKKSPTRPKRQAKPATDEGFWDCSVCTFRNSAEAFKCSICDVRKGTSTRKPRINSQLVAQQVAQQYATPPPPKKEKKEKVEKQDKEKPEKDKEISPSVTKKNTNKKTKPKSDILKDPPSEANSIQSANATTKTSETNHTSRPRLKNVDRSTAQQLAVTVGNVTVIITDFKEKTRSSSTSSSTVTSSAGSEQQNQSSSGSESTDKGSSRSSTPKGDMSAVNDESF

Specificity

RYBP - RING1 and YY1 binding protein (1F4)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for RYBP/DEDAF Antibody (1F4)

Western Blot: RYBP/DEDAF Antibody (1F4) [H00023429-M08]

Western Blot: RYBP/DEDAF Antibody (1F4) [H00023429-M08]

Western Blot: RYBP/DEDAF Antibody (1F4) [H00023429-M08] - Analysis of RYBP expression in transfected 293T cell line by RYBP monoclonal antibody (M08), clone 1F4. Lane 1: RYBP transfected lysatE (24.8 KDa). Lane 2: Non-transfected lysate.
Immunoprecipitation: RYBP/DEDAF Antibody (1F4) [H00023429-M08]

Immunoprecipitation: RYBP/DEDAF Antibody (1F4) [H00023429-M08]

Immunoprecipitation: RYBP/DEDAF Antibody (1F4) [H00023429-M08] - Analysis of RYBP transfected lysate using anti-RYBP monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RYBP rabbit polyclonal antibody.
Sandwich ELISA: RYBP/DEDAF Antibody (1F4) [H00023429-M08] - Detection limit for recombinant GST tagged RYBP is 0.3 ng/ml as a capture antibody.

Applications for RYBP/DEDAF Antibody (1F4)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RYBP/DEDAF

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. Several molecules including caspases and adaptor FADD contain DEDs. A novel protein that interacts with DED of caspase-8 and

Alternate Names

AAP1, APAP-1, Apoptin-associating protein 1, Death effector domain-associated factor, DEDAFYY1 and E4TF1-associated factor 1, RING1 and YY1 binding protein, RING1 and YY1-binding protein, ring1 interactor RYBP, YEAF1DED-associated factor, YY1 and E4TF1 associated factor 1

Entrez Gene IDs

23429 (Human)

Gene Symbol

RYBP

Additional RYBP/DEDAF Products

Product Documents for RYBP/DEDAF Antibody (1F4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RYBP/DEDAF Antibody (1F4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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