RYBP/DEDAF Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21428

Novus Biologicals, part of Bio-Techne
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NBP3-21428-100ul
NBP3-21428-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all RYBP/DEDAF Antibodies »

Product Summary for RYBP/DEDAF Antibody

Immunogen

Produced in rabbits immunized with purified, recombinant Human RYBP/DEDAF (Uniprot#: Q8N488; Met1-Phe228)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C.

Scientific Data Images for RYBP/DEDAF Antibody

RYBP/DEDAF Antibody Immunocytochemistry/Immunofluorescence: RYBP/DEDAF Antibody [NBP3-21428]

Immunocytochemistry/Immunofluorescence: RYBP/DEDAF Antibody [NBP3-21428]

Immunofluorescence staining of RYBP/DEDAF in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP3-21428 (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.
RYBP/DEDAF Antibody Immunohistochemistry-Paraffin: RYBP/DEDAF Antibody [NBP3-21428]

Immunohistochemistry-Paraffin: RYBP/DEDAF Antibody [NBP3-21428]

Immunochemical staining of human RYBP/DEDAF in human placenta formalin-fixed paraffin embedded sections with NBP3-21428 at 1:1000 dilution

Applications for RYBP/DEDAF Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunohistochemistry-Paraffin

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RYBP/DEDAF

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. Several molecules including caspases and adaptor FADD contain DEDs. A novel protein that interacts with DED of caspase-8 and

Alternate Names

AAP1, APAP-1, Apoptin-associating protein 1, Death effector domain-associated factor, DEDAFYY1 and E4TF1-associated factor 1, RING1 and YY1 binding protein, RING1 and YY1-binding protein, ring1 interactor RYBP, YEAF1DED-associated factor, YY1 and E4TF1 associated factor 1

Gene Symbol

RYBP

UniProt

Q8N488 (Human)

Additional RYBP/DEDAF Products

Product Documents for RYBP/DEDAF Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RYBP/DEDAF Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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