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S-Tag Epitope Tag Antibody (727)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43817

Novus Biologicals, part of Bio-Techne
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NBP2-43817

Key Product Details

Species Reactivity

Validated:

Epitope Tag

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 727

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for S-Tag Epitope Tag Antibody (727)

Immunogen

The immunogen used to generate this antibody corresponds to S-Tag Epitope Tag

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for S-Tag Epitope Tag Antibody (727)

Western Blot: S-Tag Epitope Tag Antibody (727) [NBP2-43817]

Western Blot: S-Tag Epitope Tag Antibody (727) [NBP2-43817]

Western Blot: S-Tag Epitope Tag Antibody (727) [NBP2-43817] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of S-tagged protein expressing 293T cells 12 % SDS-PAGE S Tag antibody [727] dilution: 1:5000.
Immunocytochemistry/ Immunofluorescence: S-Tag Epitope Tag Antibody (727) [NBP2-43817]

Immunocytochemistry/ Immunofluorescence: S-Tag Epitope Tag Antibody (727) [NBP2-43817]

Immunocytochemistry/Immunofluorescence: S-Tag Epitope Tag Antibody (727) [NBP2-43817] - S Tag antibody detects S Tag protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: S Tag stained by S Tag antibody diluted at 1:2000. Blue: Hoechst 33342 staining. Scale bar= 10 um.

Applications for S-Tag Epitope Tag Antibody (727)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: S-Tag Epitope Tag

The S-Tag epitope tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The recognized S-Tag epitope represents the amino acid sequence KETAAAKFERQHMDS derived from pancreatic ribonuclease A.

Alternate Names

KETAAAKFERQHMDS, S 7 Epitope tag

Additional S-Tag Epitope Tag Products

Product Documents for S-Tag Epitope Tag Antibody (727)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for S-Tag Epitope Tag Antibody (727)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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