S100A/B Antibody (SR3487)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-27774

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-27774-100ul
NBP3-27774-50ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR3487

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all S100A/B Antibodies »

Product Summary for S100A/B Antibody (SR3487)

Immunogen

A synthesized peptide derived from human S100A/B (Uniprot #: P23297)

Specificity

Detects endogenous levels of total S100A/B

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for S100A/B Antibody (SR3487)

Western Blot: S100A/B Antibody (SR3487) [NBP3-27774] -

Western Blot: S100A/B Antibody (SR3487) [NBP3-27774] - Western blot analysis of (1) Human skeletal muscle lysate; (2) RAW 264.7 cell lysate.

Immunohistochemistry-Paraffin: S100A/B Antibody (SR3487) [NBP3-27774] -

Immunohistochemistry-Paraffin: S100A/B Antibody (SR3487) [NBP3-27774] - Immunohistochemical analysis of paraffin-embedded human kidney

Applications for S100A/B Antibody (SR3487)

Application
Recommended Usage

Flow Cytometry

1:50

Immunohistochemistry

1:100-1:500

Immunoprecipitation

1:50

Western Blot

1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 150mM NaCl, 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: S100A/B

S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S100 protein may function in stimulation of Ca2+ induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies. Immunoreactive S100 protein localizes in the cytoplasm and nuclei of astrocytes, Schwann's cells, ependymomas and astrogliomas. Ganglion cells do not stain for S100 protein. S100 can be detected in almost all benign naevi and malignant melanocytic tumors of the skin, and in Langerhans cells in the skin.

Alternate Names

protein S100-A1, S100 alpha, S100 calcium binding protein A1, S100 calcium-binding protein A1S100, S-100 protein alpha chain, S-100 protein subunit alpha, S100 protein, alpha polypeptide, S100A, S100-alpha

Gene Symbol

S100A1

Additional S100A/B Products

Product Documents for S100A/B Antibody (SR3487)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for S100A/B Antibody (SR3487)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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