Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI6G4

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all S100A1 Antibodies »

Product Summary for S100A1 Antibody (OTI6G4) - Azide and BSA Free

Immunogen

Full length human recombinant protein of human S100A1 (NP_006262) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

10.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for S100A1 Antibody (OTI6G4) - Azide and BSA Free

Western Blot: S100A1 Antibody (OTI6G4)Azide and BSA Free [NBP2-73967]

Western Blot: S100A1 Antibody (OTI6G4)Azide and BSA Free [NBP2-73967]

Western Blot: S100A1 Antibody (OTI6G4) - Azide and BSA Free [NBP2-73967] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY S100A1.
ELISA: S100A1 Antibody (OTI6G4) - Azide and BSA Free [NBP2-73967]

ELISA: S100A1 Antibody (OTI6G4) - Azide and BSA Free [NBP2-73967]

ELISA: S100A1 Antibody (OTI6G4) - Azide and BSA Free [NBP2-73967] - Standard curve with S100A1 recombinant protein using S100A1 Capture Antibody and HRP conjugated S100A1 Detection mAb.

Applications for S100A1 Antibody (OTI6G4) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: S100A1

S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S100 protein may function in stimulation of Ca2+ induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies. Immunoreactive S100 protein localizes in the cytoplasm and nuclei of astrocytes, Schwann's cells, ependymomas and astrogliomas. Ganglion cells do not stain for S100 protein. S100 can be detected in almost all benign naevi and malignant melanocytic tumours of the skin, and in Langerhans cells in the skin.

Long Name

S100 Calcium Binding Protein A1

Alternate Names

protein S100-A1, S100 alpha, S100 calcium binding protein A1, S100 calcium-binding protein A1S100, S-100 protein alpha chain, S-100 protein subunit alpha, S100 protein, alpha polypeptide, S100A, S100-alpha

Gene Symbol

S100A1

Additional S100A1 Products

Product Documents for S100A1 Antibody (OTI6G4) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for S100A1 Antibody (OTI6G4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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