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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # S100A1/1942

Format

Azide and BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Recombinant human full-length S100A1 protein (Uniprot: P23297)

Localization

Cytoplasmic

Specificity

The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the alpha-chain (i.e. in S-100A and S-100B) but not on the beta-chain of S-100 (i.e. in S-100B). This antibody can be used to localize S-100A in various tissue sections. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, epithelial and myoepithelial cells of the breast, salivary and sweat glands. Neoplasms derived from these cells also express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein.

Marker

Melanoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Human Chromosome Location:1q21.3
Positive Control: A431 or HT-29 Cells. Brain or Melanoma

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-59615).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for S100A1 Antibody (S100A1/1942) - Azide and BSA Free

Immunohistochemistry-Paraffin: S100A1 Antibody (S100A1/1942) - Azide and BSA Free [NBP2-59617]

Immunohistochemistry-Paraffin: S100A1 Antibody (S100A1/1942) - Azide and BSA Free [NBP2-59617]

Immunohistochemistry-Paraffin: S100A1 Antibody (S100A1/1942) - Azide and BSA Free [NBP2-59617] - Formalin-fixed, paraffin-embedded human Melanoma stained with S100A1 Antibody (S100A1/1942).
Immunohistochemistry-Paraffin: S100A1 Antibody (S100A1/1942) - Azide and BSA Free [NBP2-59617]

Immunohistochemistry-Paraffin: S100A1 Antibody (S100A1/1942) - Azide and BSA Free [NBP2-59617]

Immunohistochemistry-Paraffin: S100A1 Antibody (S100A1/1942) - Azide and BSA Free [NBP2-59617] - Formalin-fixed, paraffin-embedded human Melanoma stained with S100A1 Mouse Monoclonal Antibody (S100A1/1942)
Protein Array: S100A1 Antibody (S100A1/1942) - Azide and BSA Free [NBP2-59617]

Protein Array: S100A1 Antibody (S100A1/1942) - Azide and BSA Free [NBP2-59617]

Protein Array: S100A1 Antibody (S100A1/1942) - Azide and BSA Free [NBP2-59617] - Analysis of Protein Array containing more than 19,000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary) produces when binding to a particular protein on the HuProt array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of an Ab to its intended target. An Ab is considered to specific to its intended target, if the Ab has an S-score of at least 2.5.

Applications for S100A1 Antibody (S100A1/1942) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Protein Array

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed or Frozen): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: S100A1

S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S100 protein may function in stimulation of Ca2+ induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies. Immunoreactive S100 protein localizes in the cytoplasm and nuclei of astrocytes, Schwann's cells, ependymomas and astrogliomas. Ganglion cells do not stain for S100 protein. S100 can be detected in almost all benign naevi and malignant melanocytic tumours of the skin, and in Langerhans cells in the skin.

Long Name

S100 Calcium Binding Protein A1

Alternate Names

protein S100-A1, S100 alpha, S100 calcium binding protein A1, S100 calcium-binding protein A1S100, S-100 protein alpha chain, S-100 protein subunit alpha, S100 protein, alpha polypeptide, S100A, S100-alpha

Gene Symbol

S100A1

Additional S100A1 Products

Product Documents for S100A1 Antibody (S100A1/1942) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for S100A1 Antibody (S100A1/1942) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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