S100A1 Antibody (S100A1/1942) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59617
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # S100A1/1942
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Recombinant human full-length S100A1 protein (Uniprot: P23297)
Localization
Cytoplasmic
Specificity
The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the alpha-chain (i.e. in S-100A and S-100B) but not on the beta-chain of S-100 (i.e. in S-100B). This antibody can be used to localize S-100A in various tissue sections. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, epithelial and myoepithelial cells of the breast, salivary and sweat glands. Neoplasms derived from these cells also express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein.
Marker
Melanoma Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Human Chromosome Location:1q21.3
Positive Control: A431 or HT-29 Cells. Brain or Melanoma
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-59615).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Positive Control: A431 or HT-29 Cells. Brain or Melanoma
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-59615).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for S100A1 Antibody (S100A1/1942) - Azide and BSA Free
Immunohistochemistry-Paraffin: S100A1 Antibody (S100A1/1942) - Azide and BSA Free [NBP2-59617]
Immunohistochemistry-Paraffin: S100A1 Antibody (S100A1/1942) - Azide and BSA Free [NBP2-59617] - Formalin-fixed, paraffin-embedded human Melanoma stained with S100A1 Antibody (S100A1/1942).Immunohistochemistry-Paraffin: S100A1 Antibody (S100A1/1942) - Azide and BSA Free [NBP2-59617]
Immunohistochemistry-Paraffin: S100A1 Antibody (S100A1/1942) - Azide and BSA Free [NBP2-59617] - Formalin-fixed, paraffin-embedded human Melanoma stained with S100A1 Mouse Monoclonal Antibody (S100A1/1942)Protein Array: S100A1 Antibody (S100A1/1942) - Azide and BSA Free [NBP2-59617]
Protein Array: S100A1 Antibody (S100A1/1942) - Azide and BSA Free [NBP2-59617] - Analysis of Protein Array containing more than 19,000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary) produces when binding to a particular protein on the HuProt array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of an Ab to its intended target. An Ab is considered to specific to its intended target, if the Ab has an S-score of at least 2.5.Applications for S100A1 Antibody (S100A1/1942) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Protein Array
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed or Frozen): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: S100A1
Long Name
S100 Calcium Binding Protein A1
Alternate Names
protein S100-A1, S100 alpha, S100 calcium binding protein A1, S100 calcium-binding protein A1S100, S-100 protein alpha chain, S-100 protein subunit alpha, S100 protein, alpha polypeptide, S100A, S100-alpha
Gene Symbol
S100A1
Additional S100A1 Products
Product Documents for S100A1 Antibody (S100A1/1942) - Azide and BSA Free
Product Specific Notices for S100A1 Antibody (S100A1/1942) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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