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S100A5 Antibody (S100A5/7474) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24011

Novus Biologicals, part of Bio-Techne
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NBP3-24011

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # S100A5/7474

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant fragment (around aa1-92) of human S100A5 protein (exact sequence is proprietary)

Localization

Nucleus. Cytoplasm.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Antibody without azide - store at -20 to -80C.

Scientific Data Images for S100A5 Antibody (S100A5/7474) - Azide and BSA Free

Immunohistochemistry-Paraffin: S100A5 Antibody (S100A5/7474) - Azide and BSA Free [NBP3-24011] -

Formalin-fixed, paraffin-embedded human brain stained with S100A5 antibody (S100A5/7474) - Azide and BSA Free. Inset: PBS instead of primary antibody; secondary only negative control.

Immunohistochemistry-Paraffin: S100A5 Antibody (S100A5/7474) - Azide and BSA Free [NBP3-24011] -

Formalin-fixed, paraffin-embedded human brain stained with S100A5 antibody (S100A5/7474) - Azide and BSA Free. Inset: PBS instead of primary antibody; secondary only negative control.

Applications for S100A5 Antibody (S100A5/7474) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: S100A5

S100A5 is encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein has a Ca2+ affinity 20- to 100-fold higher than the other S100 proteins studied under identical conditions. This protein also binds Zn2+ and Cu2+, and Cu2+ strongly which impairs the binding of Ca2+. This protein is expressed in very restricted regions of the adult brain.

Alternate Names

Protein S-100D, S100 calcium binding protein A5, S100 calcium-binding protein A5S100Dprotein S100-A5

Gene Symbol

S100A5

Additional S100A5 Products

Product Documents for S100A5 Antibody (S100A5/7474) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for S100A5 Antibody (S100A5/7474) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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