Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI5A10

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all S100A6 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human S100A6 (NP_055439) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

10 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for S100A6 Antibody (OTI5A10) - Azide and BSA Free

Western Blot: S100A6 Antibody (OTI5A10)Azide and BSA Free [NBP2-73969]

Western Blot: S100A6 Antibody (OTI5A10)Azide and BSA Free [NBP2-73969]

Western Blot: S100A6 Antibody (OTI5A10) - Azide and BSA Free [NBP2-73969] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY S100A6.
Immunohistochemistry: S100A6 Antibody (OTI5A10) - Azide and BSA Free [NBP2-73969]

Immunohistochemistry: S100A6 Antibody (OTI5A10) - Azide and BSA Free [NBP2-73969]

Immunohistochemistry: S100A6 Antibody (OTI5A10) - Azide and BSA Free [NBP2-73969] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: S100A6 Antibody (OTI5A10) - Azide and BSA Free [NBP2-73969]

Immunohistochemistry: S100A6 Antibody (OTI5A10) - Azide and BSA Free [NBP2-73969]

Immunohistochemistry: S100A6 Antibody (OTI5A10) - Azide and BSA Free [NBP2-73969] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Applications for S100A6 Antibody (OTI5A10) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: S100A6

S100A6 is a member of the S100 family of proteins, and may function in stimulation of Ca2+-dependent insulin release, stimulation of prolactin secretion, and exocytosis. Recent studies have found that S100A6 is induced by tumour necrosis factor-alpha via a NF-kappaB-dependent mechanism, which serves a role in limiting tumour necrosis factor-alpha-induced apoptosis by regulating p53 phosphorylation.

Long Name

S100 Calcium Binding Protein A6

Alternate Names

CACY, MLN 4, PRA

Gene Symbol

S100A6

Additional S100A6 Products

Product Documents for S100A6 Antibody (OTI5A10) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for S100A6 Antibody (OTI5A10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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