S100A8 Antibody (203)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89806

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 203

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all S100A8 Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human S100A8 (Accession#: NP_002955.2; Met1-Glu93).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for S100A8 Antibody (203)

Western Blot: S100A8 Antibody (203) [NBP2-89806]

Western Blot: S100A8 Antibody (203) [NBP2-89806]

Western Blot: S100A8 Antibody (203) [NBP2-89806] - Lane A: THP-1 Whole Cell Lysate Lane B: HL-60 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:11 kDa Observed band size:11 kDa
Immunoprecipitation: S100A8 Antibody (203) [NBP2-89806]

Immunoprecipitation: S100A8 Antibody (203) [NBP2-89806]

Immunoprecipitation: S100A8 Antibody (203) [NBP2-89806] - Lane A:0.5 mg HL-60 Whole Cell Lysate 1 uL anti-S100A8 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody:Anti-S100A8 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 11 kDaObserved band size :11 kDa

Applications for S100A8 Antibody (203)

Application
Recommended Usage

Immunoprecipitation

1-2 uL/mg of lysate

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: S100A8

MRP8 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis.

Long Name

S100 Calcium Binding Protein A8

Alternate Names

Calgranulin A, CFAG, MRP8

Gene Symbol

S100A8

Additional S100A8 Products

Product Documents for S100A8 Antibody (203)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for S100A8 Antibody (203)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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