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S100A8 Antibody (CL11171) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43984

Novus Biologicals, part of Bio-Techne
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NBP3-43984

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL11171

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P05109, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for S100A8 Antibody (CL11171) - Azide and BSA Free

S100A8 Antibody (CL11171) - Azide and BSA Free Immunohistochemistry-Paraffin: S100A8 Antibody [NBP3-43984]

Immunohistochemistry-Paraffin: S100A8 Antibody [NBP3-43984]

Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.
S100A8 Antibody (CL11171) - Azide and BSA Free Immunohistochemistry-Paraffin: S100A8 Antibody [NBP3-43984]

Immunohistochemistry-Paraffin: S100A8 Antibody [NBP3-43984]

Analysis in human bone marrow and skeletal muscle tissues using NBP3-43984 antibody. Corresponding S100A8 RNA-seq data are presented for the same tissues.
S100A8 Antibody (CL11171) - Azide and BSA Free Immunohistochemistry-Paraffin: S100A8 Antibody [NBP3-43984]

Immunohistochemistry-Paraffin: S100A8 Antibody [NBP3-43984]

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Applications for S100A8 Antibody (CL11171) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:2000 - 1:5000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: S100A8

MRP8 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis.

Long Name

S100 Calcium Binding Protein A8

Alternate Names

Calgranulin A, CFAG, MRP8

Gene Symbol

S100A8

Additional S100A8 Products

Product Documents for S100A8 Antibody (CL11171) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for S100A8 Antibody (CL11171) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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