S100A9 Antibody (MAC387) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47667
![Immunohistochemistry-Paraffin: S100A9 Antibody (MAC387) - Azide and BSA Free [NBP2-47667]](https://resources.bio-techne.com/images/products/S100A8-A9-Antibody-MRP8-7C12-4-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47667-img0002.jpg "Immunohistochemistry-Paraffin: S100A9 Antibody (MAC387) - Azide and BSA Free [NBP2-47667]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Baboon, Bovine, Canine, Equine, Feline, Goat, Guinea Pig, Monkey, Rabbit
Cited:
Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Cited:
Flow (Cell Surface)
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # MAC387
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Affinity Purified monocyte membrane preparation
Localization
Cytoplasmic
Specificity
Recognizes the L1 or Calprotectin molecule (S100A9), an intra-cytoplasmic antigen comprising of a 12kDa alpha chain and a 14kDa beta chain expressed by granulocytes, monocytes and by tissue macrophages. This monoclonal antibody reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-45295).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for S100A9 Antibody (MAC387) - Azide and BSA Free
Immunohistochemistry-Paraffin: S100A9 Antibody (MAC387) - Azide and BSA Free [NBP2-47667]
Immunohistochemistry-Paraffin: S100A8/A9 Antibody (MRP8 7C12/4) - Azide and BSA Free [NBP2-47667] - Human Tonsil stained with Macrophage L1 Protein Monoclonal Antibody (MAC387)![Flow Cytometry: S100A9 Antibody (MAC387) - Azide and BSA Free [NBP2-47667]](https://resources.bio-techne.com/images/products/S100A8-A9-Antibody-MRP8-7C12-4-Azide-and-BSA-Free-Flow-Cytometry-NBP2-47667-img0001.jpg "Flow Cytometry: S100A9 Antibody (MAC387) - Azide and BSA Free [NBP2-47667]")
Flow Cytometry: S100A9 Antibody (MAC387) - Azide and BSA Free [NBP2-47667]
Flow Cytometry: S100A8/A9 Antibody (MRP8 7C12/4) - Azide and BSA Free [NBP2-47667] - Mouse splenocytes were fixed and permeabilized with Foxp3 Fixation/Permeabilization kit. Splenocytes were incubated with Fc block for 5 mins at RT and stained with or without antibody at 1:100 dilution for 25 mins at RT. Data was acquired on a Cytoflex LX and analyzed using FlowJo 10. This image was added from customer review. Image from the Alexa Fluor 488 version of this antibody.Applications for S100A9 Antibody (MAC387) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells in 0.1 ml
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
0.5 - 1.0 ug/ml
Immunohistochemistry-Paraffin
0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: S100A9
Long Name
S100 Calcium Binding Protein A9
Alternate Names
Calgranulin B, MRP-14
Gene Symbol
S100A9
Additional S100A9 Products
Product Documents for S100A9 Antibody (MAC387) - Azide and BSA Free
Product Specific Notices for S100A9 Antibody (MAC387) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...