S100A9 Antibody (MAC387)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45295

Novus Biologicals, part of Bio-Techne
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NBP2-45295-0.02mg
NBP2-45295-0.1mg
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Baboon, Bovine, Canine, Equine, Feline, Goat, Guinea Pig, Monkey, Rabbit

Cited:

Mouse, Canine, Rabbit

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Flow - CS, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # MAC387

Concentration

0.2 mg/ml

View all S100A9 Antibodies »

Product Specifications

Immunogen

Affinity Purified monocyte membrane preparation

Localization

Cytoplasmic

Specificity

Recognizes the L1 or Calprotectin molecule (S100A9), an intra-cytoplasmic antigen comprising of a 12kDa alpha chain and a 14kDa beta chain expressed by granulocytes, monocytes and by tissue macrophages. This monoclonal antibody reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47667)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for S100A9 Antibody (MAC387)

Immunohistochemistry-Paraffin: S100A9 Antibody (MAC387) [NBP2-45295]

Immunohistochemistry-Paraffin: S100A9 Antibody (MAC387) [NBP2-45295]

Immunohistochemistry-Paraffin: S100A9 Antibody (MAC387) [NBP2-45295] - Human Tonsil stained with Macrophage L1 Protein Monoclonal Antibody (MAC387)
Flow Cytometry: S100A9 Antibody (MAC387) [NBP2-45295]

Flow Cytometry: S100A9 Antibody (MAC387) [NBP2-45295]

Flow Cytometry: S100A8/A9 Antibody (MRP8 7C12/4) [NBP2-45295] - Mouse splenocytes were fixed and permeabilized with Foxp3 Fixation/Permeabilization kit. Splenocytes were incubated with Fc block for 5 mins at RT and stained with or without antibody at 1:100 dilution for 25 mins at RT. Data was acquired on a Cytoflex LX and analyzed using FlowJo 10. This image was added from customer review. Image from the Alexa Fluor 488 version of this antibody.

Applications for S100A9 Antibody (MAC387)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

0.5 - 1.0 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: S100A9

S100A9 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A9 may function in the inhibition of casein kinase, and altered expression is associated with the disease cystic fibrosis. Overexpression of S100A9 has also recently been linked to a poor prognosis with invasive ductal carcinoma of the breast.

Long Name

S100 Calcium Binding Protein A9

Alternate Names

Calgranulin B, MRP-14

Gene Symbol

S100A9

UniProt

P05109, P06702

Additional S100A9 Products

Product Documents for S100A9 Antibody (MAC387)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for S100A9 Antibody (MAC387)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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